产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8029, RRID:AB_2840092.
展开/折叠
PKU beta; PKU-beta; serine threonine protein kinase; Serine/threonine protein kinase tousled like 1; Serine/threonine-protein kinase tousled-like 1; SNARE protein kinase SNAK; Tlk1; TLK1_HUMAN; Tousled like kinase 1; Tousled-like kinase 1;
抗原和靶标
Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
- Q9UKI8 TLK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVQSSSGSLEGPPSWSQLSTSPTPGSAAAARSLLNHTPPSGRPREGAMDELHSLDPRRQELLEARFTGVASGSTGSTGSCSVGAKASTNNESSNHSFGSLGSLSDKESETPEKKQSESSRGRKRKAENQNESSQGKSIGGRGHKISDYFEYQGGNGSSPVRGIPPAIRSPQNSHSHSTPSSSVRPNSPSPTALAFGDHPIVQPKQLSFKIIQTDLTMLKLAALESNKIQDLEKKEGRIDDLLRANCDLRRQIDEQQKLLEKYKERLNKCISMSKKLLIEKSTQEKLSSREKSMQDRLRLGHFTTVRHGASFTEQWTDGFAFQNLVKQQEWVNQQREDIERQRKLLAKRKPPTANNSQAPSTNSEPKQRKNKAVNGAENDPFVRPNLPQLLTLAEYHEQEEIFKLRLGHLKKEEAEIQAELERLERVRNLHIRELKRINNEDNSQFKDHPTLNERYLLLHLLGRGGFSEVYKAFDLYEQRYAAVKIHQLNKSWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDTFCTVLEYCEGNDLDFYLKQHKLMSEKEARSIVMQIVNALRYLNEIKPPIIHYDLKPGNILLVDGTACGEIKITDFGLSKIMDDDSYGVDGMDLTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFFQCLYGRKPFGHNQSQQDILQENTILKATEVQFPVKPVVSSEAKAFIRRCLAYRKEDRFDVHQLANDPYLLPHMRRSNSSGNLHMAGLTASPTPPSSSIITY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UKI8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S22 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| K234 | Acetylation | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| T688 | Phosphorylation | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| S741 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S744 | Phosphorylation | Uniprot | |
| S760 | Phosphorylation | Uniprot |
研究背景
Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly. Isoform 3 phosphorylates and enhances the stability of the t-SNARE SNAP23, augmenting its assembly with syntaxin. Isoform 3 protects the cells from the ionizing radiation by facilitating the repair of DSBs. In vitro, phosphorylates histone H3 at 'Ser-10'.
Nucleus.
Widely expressed. Present in fetal placenta, liver, kidney and pancreas but not heart or skeletal muscle. Also found in adult cell lines. Isoform 3 is ubiquitously expressed in all tissues examined.
Heterodimerizes with TLK2. Interacts with ASF1A and ASF1B.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
限制条款
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