产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8034, RRID:AB_2840097.
展开/折叠
bHLHd4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; Helix loop helix zipper protein; MAX protein; MGC10775; MGC11225; MGC18164; MGC34679; MGC36767; MYC associated factor X; Myc binding novel HLH/LZ protein; Orf 1; Orf1; Protein max;
抗原和靶标
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.
- P61244 MAX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61244 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S11 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K24 | Ubiquitination | Uniprot | |
| K40 | Acetylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K66 | Acetylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| Y73 | Phosphorylation | Uniprot | |
| R75 | Methylation | Uniprot | |
| K89 | Methylation | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| Y123 | Phosphorylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K153 | Acetylation | Uniprot | |
| K154 | Acetylation | Uniprot |
研究背景
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements.
Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes.
Nucleus. Cell projection>Dendrite.
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MYC or MAD. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with SPAG9. The heterodimer MYC:MAX interacts with ABI1; the interaction may enhance MYC:MAX transcriptional activity.
Belongs to the MAX family.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
限制条款
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