| 产品: | 磷酸化 RBL2 (Ser952) 抗体 |
| 货号: | AF8036 |
| 描述: | Rabbit polyclonal antibody to Phospho-RBL2 (Ser952) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 128kDa; 128kD(Calculated). |
| 蛋白号: | Q08999 |
| RRID: | AB_2840099 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8036, RRID:AB_2840099.
展开/折叠
130 kDa retinoblastoma associated protein; 130 kDa retinoblastoma-associated protein; IB4; p130; PPAR-alpha-interacting complex protein 128; PRB2; Rb2; Rbl2; RBL2_HUMAN; RBR 2; RBR-2; RBR2; Retinoblastoma like 2; Retinoblastoma-like protein 2; Retinoblastoma-related protein 2; RIC128;
抗原和靶标
- Q08999 RBL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDEAARAEAWDSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTVSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLCSLHDGLVLEAKGIKEHFWKPYIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDERIFLGEDAEEEIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVSTATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNENRVPTCEEVMPPQNLERADEICIAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFFRKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQPSSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYPFVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGSH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q08999 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S98 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| T115 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K393 | Ubiquitination | Uniprot | |
| T401 | Phosphorylation | P11802 (CDK4) , Q00534 (CDK6) | Uniprot |
| T404 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T417 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K443 | Ubiquitination | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| Y544 | Phosphorylation | Uniprot | |
| S603 | Phosphorylation | Uniprot | |
| S639 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T642 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T647 | Phosphorylation | Uniprot | |
| S659 | Phosphorylation | Q9HC98 (NEK6) | Uniprot |
| T661 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T664 | Phosphorylation | Uniprot | |
| T665 | Phosphorylation | Uniprot | |
| Y667 | Phosphorylation | Uniprot | |
| Y670 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Q00534 (CDK6) , P11802 (CDK4) | Uniprot |
| S676 | Phosphorylation | Uniprot | |
| T678 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S690 | Phosphorylation | Uniprot | |
| T694 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S941 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S952 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T954 | Phosphorylation | Uniprot | |
| T961 | Phosphorylation | Uniprot | |
| S962 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| S971 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Uniprot | |
| S973 | Phosphorylation | Uniprot | |
| T974 | Phosphorylation | Uniprot | |
| S981 | Phosphorylation | Uniprot | |
| S982 | Phosphorylation | Uniprot | |
| T986 | Phosphorylation | Uniprot | |
| T988 | Phosphorylation | Uniprot | |
| S995 | Phosphorylation | Uniprot | |
| T1019 | Phosphorylation | Uniprot | |
| S1035 | Phosphorylation | P11802 (CDK4) , Q00534 (CDK6) | Uniprot |
| Y1037 | Phosphorylation | Uniprot | |
| T1042 | Phosphorylation | Uniprot | |
| S1044 | Phosphorylation | P24941 (CDK2) | Uniprot |
| Y1057 | Phosphorylation | Uniprot | |
| S1059 | Phosphorylation | Uniprot | |
| T1065 | Phosphorylation | Uniprot | |
| S1068 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1078 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1082 | Phosphorylation | Uniprot | |
| S1090 | Phosphorylation | Uniprot | |
| T1097 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1112 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S1138 | Phosphorylation | Uniprot |
研究背景
Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
Nucleus.
Interacts with AATF. Interacts with KMT5B, KMT5C and USP4 (By similarity). Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with RINT1. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4 and isoform PML-5). Interacts with RBBP9 (By similarity).
Belongs to the retinoblastoma protein (RB) family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.