Phospho-CD130/gp130 (Ser782) Antibody - #AF8038

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产品: 磷酸化 CD130/gp130 (Ser782) 抗体
货号: AF8038
描述: Rabbit polyclonal antibody to Phospho-CD130/gp130 (Ser782)
应用: WB IHC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 160kDa; 104kD(Calculated).
蛋白号: P40189
RRID: AB_2840101

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-CD130/gp130 (Ser782) Antibody detects endogenous levels of CD130/gp130 only when phosphorylated at Ser782.
RRID:
AB_2840101
引用格式: Affinity Biosciences Cat# AF8038, RRID:AB_2840101.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD130; CD130 antigen; CDw130; gp130; GP130 RAPS; IL 6R beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; IL6 ST; IL6RB_HUMAN; IL6ST; Interleukin 6 receptor subunit beta; Interleukin receptor beta chain; Interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; Membrane glycoprotein gp130; Oncostatin M receptor; Oncostatin M receptor alpha subunit; Oncostatin-M receptor subunit alpha;

抗原和靶标

免疫原:
Uniprot:

P40189(IL6RB_HUMAN) >>Visit HPA database.

基因/基因ID:
IL6ST(3572)
表达:
P40189 IL6RB_HUMAN:

Found in all the tissues and cell lines examined (PubMed:2261637). Expression not restricted to IL6 responsive cells (PubMed:2261637). Expressed in blood serum (at protein level) (PubMed:24629561).

序列:
MLTLQTWLVQALFIFLTTESTGELLDPCGYISPESPVVQLHSNFTAVCVLKEKCMDYFHVNANYIVWKTNHFTIPKEQYTIINRTASSVTFTDIASLNIQLTCNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVTLTRWKSHLQNYTVNATKLTVNLTNDRYLATLTVRNLVGKSDAAVLTIPACDFQATHPVMDLKAFPKDNMLWVEWTTPRESVKKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESKCYLITVTPVYADGPGSPESIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRTIIGNETAVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIEAIVVPVCLAFLLTTLLGVLFCFNKRDLIKKHIWPNVPDPSKSHIAQWSPHTPPRHNFNSKDQMYSDGNFTDVSVVEIEANDKKPFPEDLKSLDLFKKEKINTEGHSSGIGGSSCMSSSRPSISSSDENESSQNTSSTVQYSTVVHSGYRHQVPSVQVFSRSESTQPLLDSEERPEDLQLVDHVDGGDGILPRQQYFKQNCSQHESSPDISHFERSKQVSSVNEEDFVRLKQQISDHISQSCGSGQMKMFQEVSAADAFGPGTEGQVERFETVGMEAATDEGMPKSYLPQTVRQGGYMPQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P40189 作为底物

Site PTM Type Enzyme
N43 N-Glycosylation
N83 N-Glycosylation
N131 N-Glycosylation
K140 Ubiquitination
N157 N-Glycosylation
K168 Ubiquitination
N227 N-Glycosylation
Y309 Phosphorylation
Y322 Phosphorylation
N379 N-Glycosylation
N383 N-Glycosylation
N390 N-Glycosylation
Y396 Phosphorylation
N553 N-Glycosylation
N564 N-Glycosylation
K649 Ubiquitination
S661 Phosphorylation
S667 Phosphorylation
T670 Phosphorylation
S678 Phosphorylation
Y683 Phosphorylation
K702 Ubiquitination
S710 Phosphorylation
K715 Ubiquitination
K718 Ubiquitination
Y759 Phosphorylation
T761 Phosphorylation
Y767 Phosphorylation
S780 Phosphorylation
S782 Phosphorylation
T783 Phosphorylation
Y814 Phosphorylation
S820 Phosphorylation
S824 Phosphorylation
S825 Phosphorylation
S829 Phosphorylation
K835 Ubiquitination
S838 Phosphorylation
S839 Phosphorylation
K849 Methylation
K849 Ubiquitination
S853 Phosphorylation
T890 Phosphorylation Q05655 (PRKCD)
T897 Phosphorylation
Y905 Phosphorylation
T909 Phosphorylation Q05655 (PRKCD)
Y915 Phosphorylation

翻译修饰 - P40189 作为激酶

Substrate Site Source
O60674 (JAK2) Y1007 Uniprot
O60674 (JAK2) Y1008 Uniprot

研究背景

功能:

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases. That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3. Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (By similarity). Has a role in embryonic development (By similarity). Does not bind IL6. Essential for survival of motor and sensory neurons and for differentiation of astrocytes (By similarity). Required for expression of TRPA1 in nociceptive neurons (By similarity). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (By similarity). Required for normal trabecular bone mass and cortical bone composition (By similarity).

翻译修饰:

Phosphorylation of Ser-782 down-regulates cell surface expression.

Heavily N-glycosylated. Glycosylation is required for protein stability and localization in plasma membrane but not for ligand binding.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. Expressed in blood serum (at protein level).

亚基结构:

Component of a hexamer of two molecules each of IL6, IL6R and IL6ST. Forms heterodimers composed of LIFR and IL6ST (type I OSM receptor) which are activated by LIF and OSM. Also forms heterodimers composed of OSMR and IL6ST (type II receptor) which are activated by OSM but not by LIF. Homodimer. Interacts with HCK. Interacts with INPP5D/SHIP1 (By similarity).

(Microbial infection) The homodimer binds two molecules of herpes virus 8/HHV-8 protein vIL-6.

蛋白家族:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 2 subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

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