产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8041, RRID:AB_2840104.
展开/折叠
c-fes/fps protein; cFES; Feline sarcoma (Snyder Theilen) viral (v fes)/Fujinami avian sarcoma (PRCII) viral (v fps) oncogene homolog; feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami; Feline sarcoma oncogene; Feline sarcoma/Fujinami avian sarcoma oncogene homolog; fes; FES proto oncogene, tyrosine kinase; FES_HUMAN; FPS; Oncogene FES; Oncogene FES feline sarcoma virus; P93c fes; Proto oncogene tyrosine protein kinase Fes/Fps; Proto-oncogene c-Fes; Proto-oncogene c-Fps; proto-oncogene tyrosine-protein kinase Fes/Fps; Tyrosine-protein kinase Fes/Fps;
抗原和靶标
Widely expressed. Detected in adult colon epithelium (at protein level) (PubMed:16455651, PubMed:19051325). Expressed in melanocytes (at protein level) (PubMed:28463229).
- P07332 FES_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGFSSELCSPQGHGVLQQMQEAELRLLEGMRKWMAQRVKSDREYAGLLHHMSLQDSGGQSRAISPDSPISQSWAEITSQTEGLSRLLRQHAEDLNSGPLSKLSLLIRERQQLRKTYSEQWQQLQQELTKTHSQDIEKLKSQYRALARDSAQAKRKYQEASKDKDRDKAKDKYVRSLWKLFAHHNRYVLGVRAAQLHHQHHHQLLLPGLLRSLQDLHEEMACILKEILQEYLEISSLVQDEVVAIHREMAAAAARIQPEAEYQGFLRQYGSAPDVPPCVTFDESLLEEGEPLEPGELQLNELTVESVQHTLTSVTDELAVATEMVFRRQEMVTQLQQELRNEEENTHPRERVQLLGKRQVLQEALQGLQVALCSQAKLQAQQELLQTKLEHLGPGEPPPVLLLQDDRHSTSSSEQEREGGRTPTLEILKSHISGIFRPKFSLPPPLQLIPEVQKPLHEQLWYHGAIPRAEVAELLVHSGDFLVRESQGKQEYVLSVLWDGLPRHFIIQSLDNLYRLEGEGFPSIPLLIDHLLSTQQPLTKKSGVVLHRAVPKDKWVLNHEDLVLGEQIGRGNFGEVFSGRLRADNTLVAVKSCRETLPPDLKAKFLQEARILKQYSHPNIVRLIGVCTQKQPIYIVMELVQGGDFLTFLRTEGARLRVKTLLQMVGDAAAGMEYLESKCCIHRDLAARNCLVTEKNVLKISDFGMSREEADGVYAASGGLRQVPVKWTAPEALNYGRYSSESDVWSFGILLWETFSLGASPYPNLSNQQTREFVEKGGRLPCPELCPDAVFRLMEQCWAYEPGQRPSFSTIYQELQSIRKRHR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P07332 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S64 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| Y261 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| T421 | Phosphorylation | Uniprot | |
| T423 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| Y461 | Phosphorylation | Uniprot | |
| S477 | Phosphorylation | Uniprot | |
| T595 | Phosphorylation | Uniprot | |
| Y614 | Phosphorylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| Y713 | Phosphorylation | P07332 (FES) | Uniprot |
| S716 | Phosphorylation | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| Y734 | Phosphorylation | Uniprot | |
| K775 | Ubiquitination | Uniprot | |
| Y811 | Phosphorylation | P07332 (FES) | Uniprot |
翻译修饰 - P07332 作为激酶
研究背景
Tyrosine-protein kinase that acts downstream of cell surface receptors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, cell attachment and cell spreading. Plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Acts down-stream of the activated FCER1 receptor and the mast/stem cell growth factor receptor KIT. Plays a role in the regulation of mast cell degranulation. Plays a role in the regulation of cell differentiation and promotes neurite outgrowth in response to NGF signaling. Plays a role in cell scattering and cell migration in response to HGF-induced activation of EZR. Phosphorylates BCR and down-regulates BCR kinase activity. Phosphorylates HCLS1/HS1, PECAM1, STAT3 and TRIM28.
Autophosphorylated on Tyr-713. Phosphorylated by LYN in response to FCER1 activation. Phosphorylated by HCK.
Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle. Golgi apparatus. Cell junction>Focal adhesion.
Note: Distributed throughout the cytosol when the kinase is not activated. Association with microtubules requires activation of the kinase activity. Shuttles between focal adhesions and cell-cell contacts in epithelial cells. Recruited to the lateral cell membrane in polarized epithelial cells by interaction with phosphorylated EZR. Detected at tubular membrane structures in the cytoplasm and at the cell periphery.
Widely expressed. Detected in adult colon epithelium (at protein level). Expressed in melanocytes (at protein level).
Homooligomer. Interacts with BCR. Interacts (when activated, via coiled coil domain) with TRIM28. Interacts (via SH2 domain) with phosphorylated EZR, MS4A2/FCER1B and HCLS1/HS1. Interacts with phosphorylated KIT. Interacts with FLT3. Interacts (via F-BAR domain) with soluble tubulin. Interacts (via SH2 domain) with microtubules.
The coiled coil domains are important for regulating the kinase activity. They mediate homooligomerization and probably also interaction with other proteins.
The N-terminal region including the first coiled coil domain mediates interaction with phosphoinositide-containing membranes.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
研究领域
· Organismal Systems > Development > Axon guidance. (View pathway)
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