Phospho-DAPK1 (Ser308) Antibody - #AF8046

Figure 7 A DAPK1 inhibitor prevents the PTBP1 knockdown induced reprogramming. (A-E) Western blot study of NTN1, DAPK1, P-DAPK1(Ser308), P-P53(ser20), and caspase 3(p17) protein in U251 cells infected with sh-Luci and sh-PTBP1 for 3 and 7 days. (n = 3). As an internal reference protein, GAPDH was used. (F) Activity of PP2A during reprogramming. (n = 3). (G-I) TUJ1 and KI67 positive rates of six distinct groups constituted of lentivirus (sh-Luci or sh-PTBP1) and TC-DAPK6 (vehicle, 100 nM, 250 nM, and 500 nM; nine random fields from triplicate samples were recorded for quantification; TUJ1+ (%) = TUJ1+ M-cherry+/M-cherry+; KI67+ (%) = KI67+ M-cherry+/M-cherry+; M-cherry+ cells = 156-553 for each condition). The data are presented as mean ± SD. *P < 0.05, *** P < 0.001 vs. sh-Luci-3d or sh-PTBP1 + vehicle group. Dpi (d): days post infection; ND: not detected; NS: no significance. Scale: 100 µm.

Figure 8. Effects of 2-BFI on tMCAO-induced changes in p-DAPK1. (A)Immunofluorescence staining of p-DAPK1 in cerebral cortex(scale bar = 20 um). Red cells are p-DAPK1positive. Magnification, 400X. (B) Ratio of p-DAPK1 positive cell of tMCAO rats treated with saline, 2-BFI,NVP-AAM077,Ifenprodil, 2-BFI+NVP-AAM077,or 2-BFI + Ifenprodil (n=5). All data are mean ± SEM. * p< 0.05, ** p< 0.01, *** p < 0.001, n.s., not significant.