Phospho-Smad2 (Ser255) Antibody - #AF8059

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产品: 磷酸化 Smad2 (Ser255) 抗体
货号: AF8059
描述: Rabbit polyclonal antibody to Phospho-Smad2 (Ser255)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: Q15796
RRID: AB_2840122

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Smad2 (Ser255) Antibody detects endogenous levels of Smad2 only when phosphorylated at Ser255.
RRID:
AB_2840122
引用格式: Affinity Biosciences Cat# AF8059, RRID:AB_2840122.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Drosophila, homolog of, MADR2; hMAD-2; HsMAD2; JV18; JV18-1; JV181; MAD; MAD homolog 2; MAD Related Protein 2; Mad-related protein 2; MADH2; MADR2; MGC22139; MGC34440; Mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic, Drosophila, homolog of, 2; Mothers against DPP homolog 2; OTTHUMP00000163489; Sma and Mad related protein 2; Sma- and Mad-related protein 2 MAD; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2; SMAD2_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Smad2 around the phosphorylation site of Ser255.

基因/基因ID:
SMAD2(4087)

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Squalene epoxidase promotes hepatocellular carcinoma development by activating STRAP transcription and TGF-β/SMAD signalling. British journal of pharmacology, 2023 (PubMed: 36581319) [IF=6.8]

Application: WB    Species: Human    Sample: Huh7 and SMMC7721 cells

FIGURE 5 SQLE promotes metastasis by activating the TGF-β/SMAD signalling pathway in HCC. (a) Heatmap shows differentially expressed genes in Huh7 cells with or without SQLE knockdown. (b,c) KEGG and GO enrichment analysis of DEGs in Huh7 cells (NC vs. siSQLE#2). (d,e) The protein expression of SMAD2/3 and p-SMAD2/3 in Huh7 and SMMC7721 cells transfected with siSQLE#2 or HA-SQLE plasmid was determined by western blot analysis. GAPDH was used as the loading control. n = 5. (f,g) Huh7 and SMMC7721 cells were treated with siSQLE#2 for 36 h in the presence of TGF-β1 (5 ng/ml) for 12 h. Wound healing assays and migration assays were used to evaluate cell metastasis viability. n = 5. (h) qRT–PCR showed the changes in EMT markers after treatment with TGF-β1 and siSQLE#2. n = 5. (i) SQLE-siRNA reversed the up-regulation effect of TGF-β1 on TGF/SMAD signalling pathway proteins. Tubulin was used as the loading control. (j–l) Huh7 and SMMC7721 cells were treated with HA-SQLE for 36 h with or without SB-431542 (5 μM) for 24 h. n = 5. qRT-PCR (j) showed the changes in EMT markers, and migration assays (k) and wound healing assays (l) evaluated cell metastasis viability. n = 5. *P 
2). Squalene epoxidase promotes hepatocellular carcinoma development by activating STRAP transcription and TGF-β/SMAD signalling. British Journal of Pharmacology, 2023 (PubMed: 36581319) [IF=6.8]

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