Phospho-Syndecan 4 (Ser179) Antibody - #AF8061

FIGURE 3 Dephosphorylation of SDC4 increased SDC4 phase sepration and the recruitment of syntenin on the plasma membrane (PM). (A) Confocal laser scanning microscopy (CLSM) images of Staurosporine and PMA regulate SDC4 clusters on the PM of HeLa cells. An anti-SDC4-ecto antibody and a Cy3-labelled secondary antibody were used to detect the location of SDC4. (B) SDC4-eGFP-syntenin cells were stimulated with PKC-activating phorbol ester (PMA) and staurosporine. Dimethyl sulfoxide (DMSO) was used as the control. (C) For the quantification of the subcellular localization of syntenin stimulated with PMA or staurosporine, the number of cells that have syntenin in the cytoplasm and at the plasma membrane was analysed. The y-axis represents the ratio of the number of cells that have syntenin on the membrane to those in the cytoplasm. The results were calculated in 3 independent experiments (**p