| 产品: | 磷酸化 Hic-5 (Tyr60) 抗体 |
| 货号: | AF8064 |
| 描述: | Rabbit polyclonal antibody to Phospho-Hic-5 (Tyr60) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 50kDa; 50kD(Calculated). |
| 蛋白号: | O43294 |
| RRID: | AB_2840127 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8064, RRID:AB_2840127.
展开/折叠
Androgen receptor coactivator 55 kDa protein; Androgen receptor coactivator ARA55; Androgen receptor-associated protein of 55 kDa; ARA55; Hic-5; Hydrogen peroxide-inducible clone 5 protein; Tgfb1i1; TGFI1_HUMAN; Transforming growth factor beta 1 induced transcript 1; Transforming growth factor beta-1-induced transcript 1 protein; TSC 5;
抗原和靶标
Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).
- O43294 TGFI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDLDALLSDLETTTSHMPRSGAPKERPAEPLTPPPSYGHQPQTGSGESSGASGDKDHLYSTVCKPRSPKPAAPAAPPFSSSSGVLGTGLCELDRLLQELNATQFNITDEIMSQFPSSKVASGEQKEDQSEDKKRPSLPSSPSPGLPKASATSATLELDRLMASLSDFRVQNHLPASGPTQPPVVSSTNEGSPSPPEPTGKGSLDTMLGLLQSDLSRRGVPTQAKGLCGSCNKPIAGQVVTALGRAWHPEHFVCGGCSTALGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHKMVTALGTHWHPEHFCCVSCGEPFGDEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHPDCFVCRECFAPFSGGSFFEHEGRPLCENHFHARRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRPLTKGSFQERAGKPYCQPCFLKLFG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43294 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| T33 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| Y38 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Q14289 (PTK2B) , P06241 (FYN) | Uniprot |
| S61 | Phosphorylation | Uniprot | |
| T62 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S143 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| T407 | Phosphorylation | Uniprot | |
| S418 | Phosphorylation | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K458 | Ubiquitination | Uniprot |
研究背景
Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.
Phosphorylated by gonadotropin-releasing hormone-activated SRC.
Cell junction>Focal adhesion. Nucleus matrix. Cytoplasm>Cytoskeleton.
Note: Associated with the actin cytoskeleton; colocalizes with stress fibers.
Expressed in platelets, smooth muscle and prostate stromal cells (at protein level).
Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7. Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin. Interacts (via LIM zinc-binding domain 2) with CBLC (via RING-type zinc finger); the interaction is direct and enhances CBLC E3 ubiquitin-protein ligase activity.
The LIM zinc-binding domains mediate glucocorticoid receptor coactivation and interaction with AR, CRIP2, ILK, LIMS1, NR3C1, PPARG, TCF3, TCF7L2, SLC6A3 and SMAD3. The LIM zinc-binding 2 and LIM zinc-binding 3 domains mediate targeting to focal adhesions and actin stress fibers. The LIM zinc-binding 3 and LIM zinc-binding 4 domains mediate interaction with TRAF4 and MAPK15. The LIM zinc-binding 4 domain mediates interaction with HSPB1, homooligomerization and targeting to the nuclear matrix. The LIM zinc-binding 3 domain mediates interaction with PTPN12.
The LD (leucine and aspartate-rich) motif 3 mediates interaction with GIT1 and functions as a nuclear export signal.
Belongs to the paxillin family.
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