Phospho-Centaurin beta-1 (Ser554) Antibody - #AF8083
| 产品: | 磷酸化 Centaurin beta-1 (Ser554) 抗体 |
| 货号: | AF8083 |
| 描述: | Rabbit polyclonal antibody to Phospho-Centaurin beta-1 (Ser554) |
| 应用: | WB |
| 反应: | Human, Mouse |
| 分子量: | 81KD; 82kD(Calculated). |
| 蛋白号: | Q15027 |
| RRID: | AB_2840146 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8083, RRID:AB_2840146.
展开/折叠
ACAP 1; ACAP1; ACAP1_HUMAN; Arf GAP with coiled coil ANK repeat and PH domains 1; Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1; Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; ArfGAP with coiled-coil, ankyrin repeat and PH domains 1; CENT B1; Centaurin beta 1; Centaurin-beta-1; CENTB 1; Cnt b1; Cnt-b1; Cntb1; KIAA0050;
抗原和靶标
Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
- Q15027 ACAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL
翻译修饰 - Q15027 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Acetylation | Uniprot | |
| K4 | Ubiquitination | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K274 | Acetylation | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| K495 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| K545 | Ubiquitination | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K558 | Ubiquitination | Uniprot | |
| T711 | Phosphorylation | Uniprot | |
| Y712 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| K729 | Ubiquitination | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| T739 | Phosphorylation | Uniprot |
研究背景
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane. Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1. Interacts with ITGB1; required for ITGB1 recycling.
PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. Only one PH domain of one ACAP1 dimer inserts into the membrane, while the other PH domain acts primaryly to interact with adjacent ACAP1 dimers.
The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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