| 产品: | 磷酸化 POU2F1/OCT1 (Ser385) 抗体 |
| 货号: | AF8088 |
| 描述: | Rabbit polyclonal antibody to Phospho-POU2F1/OCT1 (Ser385) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 76KD; 76kD,51kD(Calculated). |
| 蛋白号: | P14859 | P09086 |
| RRID: | AB_2840151 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8088, RRID:AB_2840151.
展开/折叠
FLJ42836; NF A1; NF-A1; Oct 1; Oct 1B; Oct-1; OCT1; Octamer binding protein 1; Octamer binding transcription factor 1; Octamer-binding protein 1; Octamer-binding transcription factor 1; OTF 1; OTF-1; OTF1; OTTHUMP00000032348; OTTHUMP00000032350; OTTHUMP00000032351; PO21; PO2F1; PO2F1_HUMAN; POU class 2 homeobox 1; POU domain class 2 transcription factor 1; POU domain, class 2, transcription factor 1; POU2F1; class 2; Lymphoid restricted immunoglobulin octamer binding protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; NF A2; Oct 2; Oct-2; Octamer binding transcription factor 2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; OTF2; PO2F2_HUMAN; POU domain; POU domain class 2 transcription factor 2; POU2F2; transcription factor 2;
抗原和靶标
Ubiquitous. Isoform 2 is lymphocyte-specific.
P09086 PO2F2_HUMAN:Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
- P14859 PO2F1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNNPSETSKPSMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSSLSSPSALNSPGIEGLSRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVSPVLPLTSAAVTNLSVTGTSDTTSNNTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETSTTQTTSTPLSSPLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPSLMAPSQFAAGGALLSLNPGTLSGALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASAGNSAPVASLHATSTSAESIQNSLFTVASASGAASTTTTASKAQ
- P09086 PO2F2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVHSSMGAPEIRMSKPLEAEKQGLDSPSEHTDTERNGPDTNHQNPQNKTSPFSVSPTGPSTKIKAEDPSGDSAPAAPLPPQPAQPHLPQAQLMLTGSQLAGDIQQLLQLQQLVLVPGHHLQPPAQFLLPQAQQSQPGLLPTPNLFQLPQQTQGALLTSQPRAGLPTQAVTRPTLPDPHLSHPQPPKCLEPPSHPEEPSDLEELEQFARTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAETMSVDSSLPSPNQLSSPSLGFDGLPGRRRKKRTSIETNVRFALEKSFLANQKPTSEEILLIAEQLHMEKEVIRVWFCNRRQKEKRINPCSAAPMLPSPGKPASYSPHMVTPQGGAGTLPLSQASSSLSTTVTTLSSAVGTLHPSRTAGGGGGGGGAAPPLNSIPSVTPPPPATTNSTNPSPQGSHSAIGLSGLNPSTGPGLWWNPAPYQP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P14859/P09086 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S78 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S81 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S85 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S88 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S92 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T100 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S141 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S143 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S147 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T162 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S167 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T226 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S232 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T251 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T270 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K272 | Acetylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S335 | Phosphorylation | Q9HC98 (NEK6) , Q9Y478 (PRKAB1) , Q13131 (PRKAA1) | Uniprot |
| S366 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Q13131 (PRKAA1) , P17612 (PRKACA) , P50613 (CDK7) | Uniprot |
| T445 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S192 | O-Glycosylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| S272 | O-Glycosylation | Uniprot | |
| S275 | O-Glycosylation | Uniprot | |
| T302 | O-Glycosylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| R309 | Methylation | Uniprot | |
| S324 | O-Glycosylation | Uniprot | |
| S372 | O-Glycosylation | Uniprot | |
| S390 | O-Glycosylation | Uniprot | |
| S395 | O-Glycosylation | Uniprot |
研究背景
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Phosphorylated by PRKDC.
Nucleus.
Ubiquitous. Isoform 2 is lymphocyte-specific.
Interacts with NR3C1, AR, PGR and HCFC1. Associates with the herpes simplex virus VP16-induced complex; binding to HCFC1 activates the viral transcriptional activator VP16 for association with POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes.
Belongs to the POU transcription factor family. Class-2 subfamily.
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
Cytoplasm. Nucleus.
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
Interacts with NR3C1, AR and PGR.
Belongs to the POU transcription factor family. Class-2 subfamily.
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