Phospho-A-RAF (Ser299) Antibody - #AF8132

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产品: 磷酸化 A-RAF (Ser299) 抗体
货号: AF8132
描述: Rabbit polyclonal antibody to Phospho-A-RAF (Ser299)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 68kDa; 68kD(Calculated).
蛋白号: P10398
RRID: AB_2840195

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(82%)
克隆:
Polyclonal
特异性:
Phospho-A-RAF (Ser299) Antibody detects endogenous levels of A-RAF only when phosphorylated at Ser299.
RRID:
AB_2840195
引用格式: Affinity Biosciences Cat# AF8132, RRID:AB_2840195.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

A raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; Araf; ARaf proto oncogene serine/threonine protein kinase; ARAF_HUMAN; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; Serine/threonine-protein kinase A-Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 (murine sarcoma 3611 virus);

抗原和靶标

免疫原:
Uniprot:

P10398(ARAF_HUMAN) >>Visit HPA database.

基因/基因ID:
ARAF(369)
表达:
P10398 ARAF_HUMAN:

Predominantly in urogenital tissues.

序列:
MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
88
Xenopus
82
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P10398 作为底物

Site PTM Type Enzyme
S15 Phosphorylation
T20 Phosphorylation
Y24 Phosphorylation
K28 Ubiquitination
Y42 Phosphorylation
Y155 Phosphorylation
S157 Phosphorylation
S162 Phosphorylation
T181 Phosphorylation
S186 Phosphorylation
S212 Phosphorylation
T213 Phosphorylation
S214 Phosphorylation
T215 Phosphorylation
S222 Phosphorylation
T223 Phosphorylation
T224 Phosphorylation
S229 Phosphorylation
S250 Phosphorylation
T253 Phosphorylation
S257 Phosphorylation
S259 Phosphorylation
S262 Phosphorylation
S264 Phosphorylation
S265 Phosphorylation
S269 Phosphorylation
S272 Phosphorylation
S274 Phosphorylation
K282 Ubiquitination
S283 Phosphorylation
K292 Ubiquitination
Y296 Phosphorylation
S299 Phosphorylation Q13153 (PAK1)
Y301 Phosphorylation P12931 (SRC)
Y302 Phosphorylation P12931 (SRC)
K314 Ubiquitination
T318 Phosphorylation
S320 Phosphorylation
T323 Phosphorylation
K339 Ubiquitination
S341 Phosphorylation
T344 Phosphorylation
K352 Ubiquitination
T413 Phosphorylation
Y419 Phosphorylation
K423 Ubiquitination
K431 Ubiquitination
S432 Phosphorylation
T442 Phosphorylation
T452 Phosphorylation
K454 Ubiquitination
T455 Phosphorylation
S458 Phosphorylation
Y526 Phosphorylation
S528 Phosphorylation
S532 Phosphorylation
S535 Phosphorylation
S536 Phosphorylation
K540 Ubiquitination
S547 Phosphorylation
K551 Ubiquitination
S573 Phosphorylation
K576 Ubiquitination
S576 Phosphorylation
S580 Phosphorylation
S582 Phosphorylation
S585 Phosphorylation
T589 Phosphorylation
S600 Phosphorylation

翻译修饰 - P10398 作为激酶

Substrate Site Source
Q02750 (MAP2K1) S218 Uniprot
Q02750-1 (MAP2K1) S222 Uniprot
Q15599 (SLC9A3R2) S303 Uniprot
Q92934 (BAD) S75 Uniprot
Q92934 (BAD) S99 Uniprot
Q92934 (BAD) S118 Uniprot

研究背景

功能:

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.

Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.

组织特异性:

Predominantly in urogenital tissues.

亚基结构:

Interacts with TH1L/NELFD.

蛋白家族:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

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