Phospho-CAMKK1/2 (Ser458/Ser495) Antibody - #AF8136
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8136, RRID:AB_2801648.
展开/折叠
Ca2+/calmodulin-dependent protein kinase kinase alpha; Calcium/calmodulin dependent protein kinase kinase; Calcium/calmodulin dependent protein kinase kinase 1 alpha; Calcium/calmodulin dependent protein kinase kinase 1; Calcium/calmodulin dependent protein kinase kinase alpha; Calcium/calmodulin-dependent protein kinase kinase 1; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM kinase kinase alpha; CaM-kinase IV kinase; CaM-kinase kinase 1; CaM-kinase kinase alpha; CaM-KK 1; CaM-KK alpha; CaMKK 1; CaMKK alpha; CAMKK alpha protein; CaMKK; Camkk1; CAMKKA; DKFZp761M0423; KKCC1_HUMAN; MGC34095; OTTMUSP00000006419; Calcium/calmodulin dependent protein kinase beta; Calcium/calmodulin dependent protein kinase kinase 2; Calcium/calmodulin dependent protein kinase kinase 2 beta; Calcium/calmodulin dependent protein kinase kinase beta; Calcium/calmodulin-dependent protein kinase kinase 2; Calcium/calmodulin-dependent protein kinase kinase beta; CaM kinase kinase beta; CaM KK beta; CaM-kinase kinase 2; CaM-kinase kinase beta; CaM-KK 2; CaM-KK beta; CaMKK 2; CAMKK; CaMKK beta; CAMKK beta protein; Camkk2; CAMKKB; KKCC2_HUMAN;
抗原和靶标
Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
- Q8N5S9 KKCC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEEPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLVKEGFGEGGKSPELPGVQEDEAAS
- Q96RR4 KKCC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSCVSSQPSSNRAAPQDELGGRGSSSSESQKPCEALRGLSSLSIHLGMESFIVVTECEPGCAVDLGLARDRPLEADGQEVPLDTSGSQARPHLSGRKLSLQERSQGGLAAGGSLDMNGRCICPSLPYSPVSSPQSSPRLPRRPTVESHHVSITGMQDCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRRPPPRGTRPAPGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPTLKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSNEFKGSDALLSNTVGTPAFMAPESLSETRKIFSGKALDVWAMGVTLYCFVFGQCPFMDERIMCLHSKIKSQALEFPDQPDIAEDLKDLITRMLDKNPESRIVVPEIKLHPWVTRHGAEPLPSEDENCTLVEVTEEEVENSVKHIPSLATVILVKTMIRKRSFGNPFEGSRREERSLSAPGNLLTKKPTRECESLSELKEARQRRQPPGHRPAPRGGGGSALVRGSPCVESCWAPAPGSPARMHPLRPEEAMEPE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8N5S9/Q96RR4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| T85 | Phosphorylation | Q96RR4 (CAMKK2) | Uniprot |
| S95 | Phosphorylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Q00535 (CDK5) , P49841 (GSK3B) , P49840 (GSK3A) | Uniprot |
| S132 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | P49840 (GSK3A) , P49841 (GSK3B) , Q00535 (CDK5) | Uniprot |
| S136 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | P49841 (GSK3B) , P49840 (GSK3A) , Q00535 (CDK5) | Uniprot |
| T145 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| T483 | Phosphorylation | Q96RR4 (CAMKK2) | Uniprot |
| K488 | Ubiquitination | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | P53355 (DAPK1) | Uniprot |
| S572 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| S52 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S82 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| R102 | Methylation | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| R469 | Methylation | Uniprot | |
| R472 | Methylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S492 | Phosphorylation | Uniprot |
翻译修饰 - Q8N5S9/Q96RR4 作为激酶
研究背景
Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.
Appears to be autophosphorylated in a Ca(2+)/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca(2+)/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4 (By similarity).
Cytoplasm. Nucleus.
Interacts with CAMK4 and calmodulin.
The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching.
Autophosphorylated and phosphorylated by PKA. Each isoform may show a different pattern of phosphorylation.
Nucleus. Cytoplasm. Cell projection>Neuron projection.
Note: Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction.
Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
Interacts with calmodulin.
The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Alcoholism.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
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