产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8138, RRID:AB_2840200.
展开/折叠
C SRC; C SRC kinase; C src Tyrosine Kinase; C-SRC kinase; c-src tyrosine kinase; Csk A; CSK; CSK_HUMAN; CYTOPLASMIC TYROSINE KINASE; EC 2.7.10.2; MGC112926; MGC117393; MGC154049; P60 Src; Protein tyrosine kinase CYL; Protein-tyrosine kinase CYL; Proto oncogene tyrosine protein kinase; Tyrosine protein kinase CSK; Tyrosine-protein kinase CSK; zgc:154049;
抗原和靶标
- P41240 CSK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCDGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTSDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAAMRPSFLQLREQLEHIKTHELHL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P41240 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| Y18 | Phosphorylation | Uniprot | |
| T23 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| Y97 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| Y184 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| Y304 | Phosphorylation | P41240 (CSK) | Uniprot |
| K329 | Ubiquitination | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K393 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| Y416 | Phosphorylation | P41240 (CSK) | Uniprot |
| K420 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot |
翻译修饰 - P41240 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O15492 (RGS16) | Y168 | Uniprot |
| P05412 (JUN) | Y26 | Uniprot |
| P05412 (JUN) | Y170 | Uniprot |
| P06239 (LCK) | Y505 | Uniprot |
| P06241-3 (FYN) | Y476 | Uniprot |
| P06241-2 (FYN) | Y528 | Uniprot |
| P06241 (FYN) | Y531 | Uniprot |
| P06396-2 (GSN) | Y35 | Uniprot |
| P06396-2 (GSN) | Y358 | Uniprot |
| P06396-2 (GSN) | Y414 | Uniprot |
| P06396-2 (GSN) | Y552 | Uniprot |
| P06396-2 (GSN) | Y600 | Uniprot |
| P07947 (YES1) | Y537 | Uniprot |
| P07948-1 (LYN) | Y508 | Uniprot |
| P08631-1 (HCK) | Y522 | Uniprot |
| P09769 (FGR) | Y523 | Uniprot |
| P12931-1 (SRC) | Y530 | Uniprot |
| P16284 (PECAM1) | Y713 | Uniprot |
| P41240 (CSK) | Y304 | Uniprot |
| P41240 (CSK) | Y416 | Uniprot |
| P42685 (FRK) | Y497 | Uniprot |
| P49023-2 (PXN) | Y40 | Uniprot |
| P49023-2 (PXN) | Y88 | Uniprot |
| Q8TD08-1 (MAPK15) | T175 | Uniprot |
| Q8TD08-1 (MAPK15) | Y177 | Uniprot |
研究背景
Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated.
Cytoplasm. Cell membrane.
Note: Mainly cytoplasmic, also present in lipid rafts.
Expressed in lung and macrophages.
Homodimer (via SH3-domain). Interacts with PTPN22. Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1; these interactions serve to recruit CSK to the membrane where it can phosphorylate and inhibit Src-family kinases. Interacts with SRCIN1. Interacts with RHOH. Interacts (via SH2 domain) with SCIMP; this interaction is dependent on phosphorylation of SCIMP 'Tyr-107'. Interacts (via SH2 domain) with PRAG1 (when phosphorylated at 'Tyr-391'); this interaction prevents translocation of CSK from the cytoplasm to the membrane leading to increased activity of CSK (By similarity).
The architecture of this protein is similar to that of Src-family kinases (SFKs) with one N-terminal SH3 domain, one SH2 domain, and a C-terminal kinase domain.
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
研究领域
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
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