Phospho-ERK5 (Thr219+Tyr221) Antibody - #AF8146
| 产品: | 磷酸化 ERK5 (Thr219+Tyr221) 抗体 |
| 货号: | AF8146 |
| 描述: | Rabbit polyclonal antibody to Phospho-ERK5 (Thr219+Tyr221) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 115 kDa; 88kD(Calculated). |
| 蛋白号: | Q13164 |
| RRID: | AB_2840208 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8146, RRID:AB_2840208.
展开/折叠
Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1; BMK1 Kinase; EC 2.7.11.24; ERK 4; ERK 5; ERK-5; ERK4; ERK5; Extracellular signal regulated kinase 5; Extracellular signal-regulated kinase 5; MAP kinase 7; MAPK 7; MAPK7; Mitogen activated protein kinase 7; Mitogen-activated protein kinase 7; MK07_HUMAN; OTTHUMP00000065906; OTTHUMP00000065907; PRKM 7; PRKM7; PROTEIN KINASE, MITOGEN-ACTIVATED, 7;
抗原和靶标
Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
- Q13164 MK07_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEPLKEEDGEDGSAEPPGPVKAEPAHTAASVAAKNLALLKARSFDVTFDVGDEYEIIETIGNGAYGVVSSARRRLTGQQVAIKKIPNAFDVVTNAKRTLRELKILKHFKHDNIIAIKDILRPTVPYGEFKSVYVVLDLMESDLHQIIHSSQPLTLEHVRYFLYQLLRGLKYMHSAQVIHRDLKPSNLLVNENCELKIGDFGMARGLCTSPAEHQYFMTEYVATRWYRAPELMLSLHEYTQAIDLWSVGCIFGEMLARRQLFPGKNYVHQLQLIMMVLGTPSPAVIQAVGAERVRAYIQSLPPRQPVPWETVYPGADRQALSLLGRMLRFEPSARISAAAALRHPFLAKYHDPDDEPDCAPPFDFAFDREALTRERIKEAIVAEIEDFHARREGIRQQIRFQPSLQPVASEPGCPDVEMPSPWAPSGDCAMESPPPAPPPCPGPAPDTIDLTLQPPPPVSEPAPPKKDGAISDNTKAALKAALLKSLRSRLRDGPSAPLEAPEPRKPVTAQERQREREEKRRRRQERAKEREKRRQERERKERGAGASGGPSTDPLAGLVLSDNDRSLLERWTRMARPAAPALTSVPAPAPAPTPTPTPVQPTSPPPGPVAQPTGPQPQSAGSTSGPVPQPACPPPGPAPHPTGPPGPIPVPAPPQIATSTSLLAAQSLVPPPGLPGSSTPGVLPYFPPGLPPPDAGGAPQSSMSESPDVNLVTQQLSKSQVEDPLPPVFSGTPKGSGAGYGVGFDLEEFLNQSFDMGVADGPQDGQADSASLSASLLADWLEGHGMNPADIESLQREIQMDSPMLLADLPDLQDP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13164 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S14 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| S31 | Phosphorylation | Uniprot | |
| K35 | Acetylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| Y216 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Q13163 (MAP2K5) | Uniprot |
| Y221 | Phosphorylation | Q13163 (MAP2K5) | Uniprot |
| T224 | Phosphorylation | Uniprot | |
| T280 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| S421 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| S433 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| S472 | Phosphorylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| K480 | Methylation | Uniprot | |
| K485 | Methylation | Uniprot | |
| S486 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| S496 | Phosphorylation | Q13164 (MAPK7) , Q15418 (RPS6KA1) | Uniprot |
| S562 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| T584 | Phosphorylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S731 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| T733 | Phosphorylation | Q13164 (MAPK7) , P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S754 | Phosphorylation | Uniprot | |
| S770 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| S772 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| S774 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| S776 | Phosphorylation | Uniprot | |
| S803 | Phosphorylation | Uniprot |
翻译修饰 - Q13164 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O00141-1 (SGK1) | S78 | Uniprot |
| P01100 (FOS) | S32 | Uniprot |
| P01100 (FOS) | T232 | Uniprot |
| P14921-1 (ETS1) | T38 | Uniprot |
| P17302 (GJA1) | S255 | Uniprot |
| P17302 (GJA1) | S282 | Uniprot |
| P19793-1 (RXRA) | T82 | Uniprot |
| P19793-1 (RXRA) | S260 | Uniprot |
| P29590 (PML) | S403 | Uniprot |
| P29590 (PML) | T409 | Uniprot |
| Q02078-5 (MEF2A) | T304 | Uniprot |
| Q02078-5 (MEF2A) | T311 | Uniprot |
| Q02078 (MEF2A) | T312 | Uniprot |
| Q02078 (MEF2A) | T319 | Uniprot |
| Q02078-5 (MEF2A) | S347 | Uniprot |
| Q02078 (MEF2A) | S355 | Uniprot |
| Q06413-3 (MEF2C) | S387 | Uniprot |
| Q12959 (DLG1) | S158 | Uniprot |
| Q13163 (MAP2K5) | S129 | Uniprot |
| Q13163 (MAP2K5) | S137 | Uniprot |
| Q13163 (MAP2K5) | S142 | Uniprot |
| Q13163 (MAP2K5) | S149 | Uniprot |
| Q13164 (MAPK7) | T28 | Uniprot |
| Q13164 (MAPK7) | S421 | Uniprot |
| Q13164 (MAPK7) | S433 | Uniprot |
| Q13164 (MAPK7) | S496 | Uniprot |
| Q13164 (MAPK7) | S731 | Uniprot |
| Q13164 (MAPK7) | T733 | Uniprot |
| Q13164 (MAPK7) | S770 | Uniprot |
| Q13164 (MAPK7) | S772 | Uniprot |
| Q13164 (MAPK7) | S774 | Uniprot |
| Q14814-1 (MEF2D) | S180 | Uniprot |
| Q14934 (NFATC4) | S168 | Uniprot |
| Q14934 (NFATC4) | S170 | Uniprot |
| Q92886 (NEUROG1) | S178 | Uniprot |
| Q92886 (NEUROG1) | S201 | Uniprot |
研究背景
Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
Cytoplasm. Nucleus. Nucleus>PML body.
Note: Translocates to the nucleus upon activation.
Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
Interacts with MAP2K5. Forms oligomers (By similarity). Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D (By similarity). Interacts with SGK1. Preferentially interacts with PML isoform PML-4 but shows interaction also with its other isoforms: isoform PML-1, isoform PML-2, isoform PML-3 and isoform PML-6. Interacts (via N-terminal half) with HSP90AB1-CDC37 chaperone complex in resting cells; the interaction is MAP2K5-independent and prevents MAPK7 from ubiquitination and proteasomal degradation.
The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
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