(3)
(10)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8160, RRID:AB_2840222.
展开/折叠
EC 2.7.10.2; JAK 3; JAK L; JAK-3; Jak3; JAK3 HUMAN; JAK3_HUMAN; JAKL; Janus kinase 3 (a protein tyrosine kinase, leukocyte); Janus kinase 3; Janus Kinase3; L JAK; L-JAK; Leukocyte janus kinase; LJAK; Protein tyrosine kinase leukocyte; Tyrosine protein kinase JAK3; Tyrosine-protein kinase JAK3;
抗原和靶标
In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
- P52333 JAK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPPSEETPLIPQRSCSLLSTEAGALHVLLPARGPGPPQRLSFSFGDHLAEDLCVQAAKASGILPVYHSLFALATEDLSCWFPPSHIFSVEDASTQVLLYRIRFYFPNWFGLEKCHRFGLRKDLASAILDLPVLEHLFAQHRSDLVSGRLPVGLSLKEQGECLSLAVLDLARMAREQAQRPGELLKTVSYKACLPPSLRDLIQGLSFVTRRRIRRTVRRALRRVAACQADRHSLMAKYIMDLERLDPAGAAETFHVGLPGALGGHDGLGLLRVAGDGGIAWTQGEQEVLQPFCDFPEIVDISIKQAPRVGPAGEHRLVTVTRTDNQILEAEFPGLPEALSFVALVDGYFRLTTDSQHFFCKEVAPPRLLEEVAEQCHGPITLDFAINKLKTGGSRPGSYVLRRSPQDFDSFLLTVCVQNPLGPDYKGCLIRRSPTGTFLLVGLSRPHSSLRELLATCWDGGLHVDGVAVTLTSCCIPRPKEKSNLIVVQRGHSPPTSSLVQPQSQYQLSQMTFHKIPADSLEWHENLGHGSFTKIYRGCRHEVVDGEARKTEVLLKVMDAKHKNCMESFLEAASLMSQVSYRHLVLLHGVCMAGDSTMVQEFVHLGAIDMYLRKRGHLVPASWKLQVVKQLAYALNYLEDKGLPHGNVSARKVLLAREGADGSPPFIKLSDPGVSPAVLSLEMLTDRIPWVAPECLREAQTLSLEADKWGFGATVWEVFSGVTMPISALDPAKKLQFYEDRQQLPAPKWTELALLIQQCMAYEPVQRPSFRAVIRDLNSLISSDYELLSDPTPGALAPRDGLWNGAQLYACQDPTIFEERHLKYISQLGKGNFGSVELCRYDPLGDNTGALVAVKQLQHSGPDQQRDFQREIQILKALHSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQRHRARLDASRLLLYSSQICKGMEYLGSRRCVHRDLAARNILVESEAHVKIADFGLAKLLPLDKDYYVVREPGQSPIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYCDKSCSPSAEFLRMMGCERDVPALCRLLELLEEGQRLPAPPACPAEVHELMKLCWAPSPQDRPSFSALGPQLDMLWSGSRGCETHAFTAHPEGKHHSLSFS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P52333 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S5 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| T391 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| K482 | Ubiquitination | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| Y506 | Phosphorylation | Uniprot | |
| K534 | Ubiquitination | Uniprot | |
| Y536 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| S663 | Phosphorylation | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| K748 | Acetylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| Y785 | Phosphorylation | P52333 (JAK3) | Uniprot |
| K823 | Ubiquitination | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| K855 | Ubiquitination | Uniprot | |
| K876 | Ubiquitination | Uniprot | |
| S880 | Phosphorylation | Uniprot | |
| Y886 | Phosphorylation | Uniprot | |
| Y904 | Phosphorylation | P52333 (JAK3) | Uniprot |
| Y929 | Phosphorylation | Uniprot | |
| S931 | Phosphorylation | Uniprot | |
| K935 | Ubiquitination | Uniprot | |
| Y939 | Phosphorylation | P52333 (JAK3) | Uniprot |
| S959 | Phosphorylation | Uniprot | |
| K972 | Ubiquitination | Uniprot | |
| K978 | Ubiquitination | Uniprot | |
| Y980 | Phosphorylation | P52333 (JAK3) | Uniprot |
| Y981 | Phosphorylation | P52333 (JAK3) | Uniprot |
| K1117 | Ubiquitination | Uniprot | |
| S1120 | Phosphorylation | Uniprot |
翻译修饰 - P52333 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O14939 (PLD2) | Y415 | Uniprot |
| O95644 (NFATC1) | Y371 | Uniprot |
| P23458 (JAK1) | Y1034 | Uniprot |
| P23458 (JAK1) | Y1035 | Uniprot |
| P29353-2 (SHC1) | Y410 | Uniprot |
| P29353-2 (SHC1) | Y448 | Uniprot |
| P42224 (STAT1) | Y701 | Uniprot |
| P42229 (STAT5A) | Y694 | Uniprot |
| P52333 (JAK3) | Y785 | Uniprot |
| P52333 (JAK3) | Y904 | Uniprot |
| P52333 (JAK3) | Y939 | Uniprot |
| P52333-1 (JAK3) | Y980 | Uniprot |
| P52333 (JAK3) | Y981 | Uniprot |
| Q15717 (ELAVL1) | Y63 | Uniprot |
| Q15717 (ELAVL1) | Y68 | Uniprot |
| Q15717 (ELAVL1) | Y200 | Uniprot |
| Q96LC7 (SIGLEC10) | Y597 | Uniprot |
| Q96LC7 (SIGLEC10) | Y667 | Uniprot |
| Q96LC7 (SIGLEC10) | Y691 | Uniprot |
研究背景
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
Tyrosine phosphorylated in response to IL-2 and IL-4. Dephosphorylation of Tyr-980 and Tyr-981 by PTPN2 negatively regulates cytokine-mediated signaling (Probable).
Endomembrane system>Peripheral membrane protein. Cytoplasm.
In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Specific types > Non-small cell lung cancer. (View pathway)
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
文献引用
Application: WB Species: rat Sample: DRG neurons
Application: WB Species: human Sample: BxPC-3 and PANC‑1 cells
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.