Phospho-MAP3K2 (Ser520) Antibody - #AF8166

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产品: 磷酸化 MAP3K2 (Ser520) 抗体
货号: AF8166
描述: Rabbit polyclonal antibody to Phospho-MAP3K2 (Ser520)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 70kDa; 70kD(Calculated).
蛋白号: Q9Y2U5
RRID: AB_2840228

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-MAP3K2 (Ser520) Antibody detects endogenous levels of MAP3K2 only when phosphorylated at Ser520.
RRID:
AB_2840228
引用格式: Affinity Biosciences Cat# AF8166, RRID:AB_2840228.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

M3K2_HUMAN; Map3k2; MAPK/ERK kinase kinase 2; MEK kinase 2; MEKK 2; MEKK2b; Mitogen activated protein kinase kinase kinase 2; Mitogen-activated protein kinase kinase kinase 2;

抗原和靶标

免疫原:
Uniprot:

Q9Y2U5(M3K2_HUMAN) >>Visit HPA database.

基因/基因ID:
MAP3K2(10746)
序列:
MDDQQALNSIMQDLAVLHKASRPALSLQETRKAKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLEDLRSKAKIAFGQSMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQEYNDGRKTFPRARRTQGTSLRSPVSFSPTDHSLSTSSGSSIFTPEYDDSRIRRRGSDIDNPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
92
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y2U5 作为底物

Site PTM Type Enzyme
S26 Phosphorylation
S109 Phosphorylation
S153 Phosphorylation
T158 Phosphorylation
S159 Phosphorylation
S163 Phosphorylation
S164 Phosphorylation
Y169 Phosphorylation
S239 Phosphorylation
Y240 Phosphorylation
S248 Phosphorylation
Y250 Phosphorylation
K257 Ubiquitination
K260 Methylation
T263 Phosphorylation
T283 Phosphorylation
T290 Phosphorylation
S297 Phosphorylation
S300 Phosphorylation
S302 Phosphorylation
T310 Phosphorylation
S311 Phosphorylation
S312 Phosphorylation
S314 Phosphorylation
S315 Phosphorylation
S331 Phosphorylation
T337 Phosphorylation
S344 Phosphorylation
S347 Phosphorylation
S349 Phosphorylation
K360 Ubiquitination
K385 Ubiquitination
K411 Ubiquitination
T432 Phosphorylation
K450 Ubiquitination
K485 Ubiquitination
S493 Phosphorylation
K498 Ubiquitination
S505 Phosphorylation
K506 Ubiquitination
S514 Phosphorylation
T516 Phosphorylation
S520 Phosphorylation
T522 Phosphorylation
T524 Phosphorylation
K581 Ubiquitination
K602 Ubiquitination

翻译修饰 - Q9Y2U5 作为激酶

Substrate Site Source
O14733 (MAP2K7) S271 Uniprot
O14733 (MAP2K7) T275 Uniprot

研究背景

功能:

Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.

翻译修饰:

Autophosphorylated.

Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.

细胞定位:

Cytoplasm. Nucleus.
Note: Upon EGF stimulation, translocates into the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with PKN2; the interaction activates PKN2 kinase activity in a MAP3K2-independent kinase activity (By similarity). Self-associates. Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts (via the kinase catalytic domain) with STK38. Interacts with XIAP/BIRC4.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

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