产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8173, RRID:AB_2840235.
展开/折叠
epididymis secretory protein Li 95; HEL-S-95; Mammalian STE20 like protein kinase 3; Mammalian STE20-like protein kinase 3; Mammalian STE20-like protein kinase 3 C-terminal; Mammalian STE20-like protein kinase 3 N-terminal; Mammalian sterile 20-like 3; MST-3; MST3/C; MST3/N; MST3B; serine threonine kinase 24; Serine/threonine kinase 24 (Ste20, yeast homolog); Serine/threonine-protein kinase 24 12 kDa subunit; Serine/threonine-protein kinase 24; STE20 homolog yeast; STE20 like kinase MST3; STE20-like kinase MST3; Stk24; STK24_HUMAN; STK3;
抗原和靶标
Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.
- Q9Y6E0 STK24_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDSRAQLWGLALNKRRATLPHPGGSTNLKADPEELFTKLEKIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEPGPLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLIPKNNPPTLEGNYSKPLKEFVEACLNKEPSFRPTAKELLKHKFILRNAKKTSYLTELIDRYKRWKAEQSHDDSSSEDSDAETDGQASGGSDSGDWIFTIREKDPKNLENGALQPSDLDRNKMKDIPKRPFSQCLSTIISPLFAELKEKSQACGGNLGSIEELRGAIYLAEEACPGISDTMVAQLVQRLQRYSLSGGGTSSH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6E0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T18 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K38 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| T190 | Phosphorylation | Q9Y6E0 (STK24) | Uniprot |
| T194 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| Y303 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| Y433 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | Uniprot |
翻译修饰 - Q9Y6E0 作为激酶
研究背景
Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation. Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.
Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues.
Cytoplasm. Nucleus. Membrane.
Note: The truncated form (MST3/N) translocates to the nucleus. Colocalizes with STK38L in the membrane.
Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.
Monomer. Interacts with CTTNBP2NL. Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner. Interacts with PDCD10; this interaction is required for the association of STK24 with RIPOR1.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
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