Phospho-NDR1/2 (Thr444/Thr442) Antibody - #AF8174

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产品: 磷酸化 NDR1/2 (Thr444/Thr442) 抗体
货号: AF8174
描述: Rabbit polyclonal antibody to Phospho-NDR1/2 (Thr444/Thr442)
应用: WB IHC
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 54KD; 54kD(Calculated).
蛋白号: Q15208 | Q9Y2H1
RRID: AB_2840236

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-NDR1/2 (Thr444/Thr442) Antibody detects endogenous levels of NDR1/2 only when phosphorylated at Thr444/442.
RRID:
AB_2840236
引用格式: Affinity Biosciences Cat# AF8174, RRID:AB_2840236.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

NDR 1; NDR; Ndr Ser/Thr kinase-like protein; NDR1; NDR1 protein kinase; Nuclear Dbf2 related kinase 1; nuclear Dbf2-related 1; Nuclear Dbf2-related kinase 1; Nuclear Dbf2-related protein; Serine/threonine protein kinase 38; Serine/threonine-protein kinase 38; STK 38; STK38; STK38_HUMAN; KIAA0965; NDR2; NDR2 protein kinase; nuclear Dbf2 related 2; Nuclear Dbf2 related kinase 2; Nuclear Dbf2-related kinase 2; Serine/threonine protein kinase 38 like; Serine/threonine-protein kinase 38-like; ST38L_HUMAN; Stk38l;

抗原和靶标

免疫原:
Uniprot:

Q15208(STK38_HUMAN) >>Visit HPA database.

Q9Y2H1(ST38L_HUMAN) >>Visit HPA database.

基因/基因ID:
STK38L(23012) | STK38(11329)
表达:
Q15208 STK38_HUMAN:

Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.

Q9Y2H1 ST38L_HUMAN:

Ubiquitously expressed with highest levels observed in the thymus.

序列:
MAMTGSTPCSSMSNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHARKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAERDILVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAFSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKVMNWKETLTFPPEVPISEKAKDLILRFCCEWEHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK

MAMTAGTTTTFPMSNHTRERVTVAKLTLENFYSNLILQHEERETRQKKLEVAMEEEGLADEEKKLRRSQHARKETEFLRLKRTRLGLDDFESLKVIGRGAFGEVRLVQKKDTGHIYAMKILRKSDMLEKEQVAHIRAERDILVEADGAWVVKMFYSFQDKRNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYISETVLAIDAIHQLGFIHRDIKPDNLLLDAKGHVKLSDFGLCTGLKKAHRTEFYRNLTHNPPSDFSFQNMNSKRKAETWKKNRRQLAYSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKVMNWKETLVFPPEVPISEKAKDLILRFCIDSENRIGNSGVEEIKGHPFFEGVDWEHIRERPAAIPIEIKSIDDTSNFDDFPESDILQPVPNTTEPDYKSKDWVFLNYTYKRFEGLTQRGSIPTYMKAGKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15208/Q9Y2H1 作为底物

Site PTM Type Enzyme
T4 Phosphorylation
S6 Phosphorylation P49841 (GSK3B)
T7 Phosphorylation P49841 (GSK3B)
S10 Phosphorylation P17612 (PRKACA)
S11 Phosphorylation P17612 (PRKACA)
S13 Phosphorylation
T16 Phosphorylation
K17 Ubiquitination
T26 Phosphorylation
Y31 Phosphorylation
K58 Acetylation
K62 Ubiquitination
K72 Ubiquitination
T74 Phosphorylation Q15208 (STK38)
K93 Ubiquitination
K109 Ubiquitination
K118 Ubiquitination
K122 Ubiquitination
K128 Ubiquitination
T183 Phosphorylation
K214 Ubiquitination
K227 Ubiquitination
C234 S-Nitrosylation
T235 Phosphorylation
K238 Ubiquitination
S252 Phosphorylation
S264 Phosphorylation
K265 Ubiquitination
T270 Phosphorylation
S281 Phosphorylation Q15208 (STK38)
T282 Phosphorylation
T285 Phosphorylation
Y288 Phosphorylation
K338 Ubiquitination
K354 Ubiquitination
K377 Ubiquitination
T407 Phosphorylation
K420 Ubiquitination
K434 Ubiquitination
Y443 Phosphorylation
T444 Phosphorylation P26927 (MST1) , Q15208 (STK38) , Q9Y6E0 (STK24) , Q13043 (STK4)
Y445 Phosphorylation
T452 Phosphorylation
R454 Methylation
Y460 Phosphorylation
K462 Ubiquitination
Site PTM Type Enzyme
K73 Ubiquitination
T75 Phosphorylation
K94 Ubiquitination
K110 Ubiquitination
Y116 Phosphorylation
K119 Ubiquitination
K123 Ubiquitination
K129 Ubiquitination
K215 Ubiquitination
K224 Ubiquitination
K228 Ubiquitination
T236 Phosphorylation
K239 Ubiquitination
S265 Phosphorylation
K266 Ubiquitination
S282 Phosphorylation Q9Y2H1 (STK38L)
T283 Phosphorylation
T286 Phosphorylation
Y289 Phosphorylation
K355 Ubiquitination
K378 Ubiquitination
K403 Ubiquitination
Y441 Phosphorylation
T442 Phosphorylation Q9Y6E0 (STK24)
Y443 Phosphorylation
T450 Phosphorylation
R452 Methylation
S454 Phosphorylation
K460 Ubiquitination

翻译修饰 - Q15208/Q9Y2H1 作为激酶

Substrate Site Source
P38936 (CDKN1A) S146 Uniprot
P46937 (YAP1) S61 Uniprot
P46937 (YAP1) S109 Uniprot
P46937 (YAP1) S127 Uniprot
P46937 (YAP1) S164 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q15208 (STK38) T74 Uniprot
Q15208 (STK38) S281 Uniprot
Q15208 (STK38) T444 Uniprot
Q2M2I8 (AAK1) S637 Uniprot
Q96QF0 (RAB3IP) S288 Uniprot
Q96RD6 (PANX2) S514 Uniprot
Q9BXF6 (RAB11FIP5) S307 Uniprot
Q9UBF8 (PI4KB) S277 Uniprot
Substrate Site Source
O60285 (NUAK1) T211 Uniprot
P46937 (YAP1) S127 Uniprot
Q96QF0 (RAB3IP) S288 Uniprot
Q9Y2H1 (STK38L) S282 Uniprot

研究背景

功能:

Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2.

翻译修饰:

ISGylated.

Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B.

细胞定位:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.

亚基结构:

Homodimeric S100B binds two molecules of STK38. Interacts with MOB1 and MOB2. Interacts with MAP3K1 and MAP3K2 (via the kinase catalytic domain). Forms a tripartite complex with MOBKL1B and STK3/MST2. Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4 (By similarity).

蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

功能:

Involved in the regulation of structural processes in differentiating and mature neuronal cells.

细胞定位:

Cytoplasm. Cytoplasm>Cytoskeleton. Membrane.
Note: Associated with the actin cytoskeleton. Co-localizes with STK24/MST3 in the membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed with highest levels observed in the thymus.

亚基结构:

Homodimeric S100B binds two molecules of STK38L. Interacts with MICAL1; leading to inhibit the protein kinase activity by antagonizing activation by MST1/STK4 (By similarity). Interacts with MOB1 and MOB2.

蛋白家族:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

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