Phospho-PASK (Thr1165) Antibody - #AF8179

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产品: 磷酸化 PASK (Thr1165) 抗体
货号: AF8179
描述: Rabbit polyclonal antibody to Phospho-PASK (Thr1165)
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 142KD; 143kD(Calculated).
蛋白号: Q96RG2
RRID: AB_2840241

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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MS Report
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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(88%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-PASK (Thr1165) Antibody detects endogenous levels of PASK only when phosphorylated at Thr1165.
RRID:
AB_2840241
引用格式: Affinity Biosciences Cat# AF8179, RRID:AB_2840241.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DKFZP434O051; DKFZp686P2031; hPASK; KIAA0135; MGC93882; mKIAA0135; PAS domain containing serine/threonine kinase; PAS domain containing serine/threonine protein kinase; PAS domain-containing serine/threonine-protein kinase; PAS kinase; PAS serine/threonine kinase; PAS-kinase; Pask; PASK_HUMAN; PASKIN; STK 37; STK37;

抗原和靶标

免疫原:
Uniprot:

Q96RG2(PASK_HUMAN) >>Visit HPA database.

基因/基因ID:
PASK(23178)
表达:
Q96RG2 PASK_HUMAN:

Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).

序列:
MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSAHRHLSRRNGLSRLCQSRTALSEDRWSSYCLSSLAAQNICTSKLHCPAAPEHTDPSEPRGSVSCCSLLRGLSSGWSSPLLPAPVCNPNKAIFTVDAKTTEILVANDKACGLLGYSSQDLIGQKLTQFFLRSDSDVVEALSEEHMEADGHAAVVFGTVVDIISRSGEKIPVSVWMKRMRQERRLCCVVVLEPVERVSTWVAFQSDGTVTSCDSLFAHLHGYVSGEDVAGQHITDLIPSVQLPPSGQHIPKNLKIQRSVGRARDGTTFPLSLKLKSQPSSEEATTGEAAPVSGYRASVWVFCTISGLITLLPDGTIHGINHSFALTLFGYGKTELLGKNITFLIPGFYSYMDLAYNSSLQLPDLASCLDVGNESGCGERTLDPWQGQDPAEGGQDPRINVVLAGGHVVPRDEIRKLMESQDIFTGTQTELIAGGQLLSCLSPQPAPGVDNVPEGSLPVHGEQALPKDQQITALGREEPVAIESPGQDLLGESRSEPVDVKPFASCEDSEAPVPAEDGGSDAGMCGLCQKAQLERMGVSGPSGSDLWAGAAVAKPQAKGQLAGGSLLMHCPCYGSEWGLWWRSQDLAPSPSGMAGLSFGTPTLDEPWLGVENDREELQTCLIKEQLSQLSLAGALDVPHAELVPTECQAVTAPVSSCDLGGRDLCGGCTGSSSACYALATDLPGGLEAVEAQEVDVNSFSWNLKELFFSDQTDQTSSNCSCATSELRETPSSLAVGSDPDVGSLQEQGSCVLDDRELLLLTGTCVDLGQGRRFRESCVGHDPTEPLEVCLVSSEHYAASDRESPGHVPSTLDAGPEDTCPSAEEPRLNVQVTSTPVIVMRGAAGLQREIQEGAYSGSCYHRDGLRLSIQFEVRRVELQGPTPLFCCWLVKDLLHSQRDSAARTRLFLASLPGSTHSTAAELTGPSLVEVLRARPWFEEPPKAVELEGLAACEGEYSQKYSTMSPLGSGAFGFVWTAVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHGSGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELMSLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQELCGGPVPGEAPNGQGCLHPGDPRLLTS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Dog
88
Zebrafish
88
Rabbit
88
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q96RG2 作为底物

Site PTM Type Enzyme
M1 Acetylation
S19 Phosphorylation
S40 Phosphorylation
S42 Phosphorylation
S55 Phosphorylation
S60 Phosphorylation
S104 Phosphorylation
S106 Phosphorylation
S109 Phosphorylation
S116 Phosphorylation
K132 Ubiquitination
K218 Acetylation
T307 Phosphorylation
K314 Ubiquitination
K316 Ubiquitination
S333 Phosphorylation
Y389 Phosphorylation
S390 Phosphorylation
S524 Phosphorylation
S533 Phosphorylation
K570 Ubiquitination
S579 Phosphorylation
S582 Phosphorylation
K594 Ubiquitination
S667 Phosphorylation
S670 Phosphorylation
S843 Phosphorylation
T874 Phosphorylation
K930 Ubiquitination
S939 Phosphorylation
S949 Phosphorylation
T954 Phosphorylation
K981 Ubiquitination
Y999 Phosphorylation
S1000 Phosphorylation
T1001 Phosphorylation
S1003 Phosphorylation
K1034 Ubiquitination
K1045 Ubiquitination
S1057 Phosphorylation
K1122 Ubiquitination
S1149 Phosphorylation Q96RG2 (PASK)
T1161 Phosphorylation Q96RG2 (PASK)
T1165 Phosphorylation Q96RG2 (PASK)
K1270 Ubiquitination
S1273 Phosphorylation Q96RG2 (PASK)
S1277 Phosphorylation Q96RG2 (PASK)
S1280 Phosphorylation Q96RG2 (PASK)
S1287 Phosphorylation Q96RG2 (PASK)
S1289 Phosphorylation Q96RG2 (PASK)
T1322 Phosphorylation
S1323 Phosphorylation

翻译修饰 - Q96RG2 作为激酶

Substrate Site Source
P13807 (GYS1) S641 Uniprot
P52945 (PDX1) T151 Uniprot
P62753 (RPS6) S235 Uniprot
P62753 (RPS6) S236 Uniprot
P68104 (EEF1A1) T432 Uniprot
Q96RG2 (PASK) S1149 Uniprot
Q96RG2 (PASK) T1161 Uniprot
Q96RG2 (PASK) T1165 Uniprot
Q96RG2 (PASK) S1273 Uniprot
Q96RG2 (PASK) S1277 Uniprot
Q96RG2 (PASK) S1280 Uniprot
Q96RG2 (PASK) S1287 Uniprot
Q96RG2 (PASK) S1289 Uniprot

研究背景

功能:

Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.

翻译修饰:

Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids.

细胞定位:

Cytoplasm. Nucleus.
Note: Localizes in the nucleus of testis germ cells and in the midpiece of sperm tails.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails (at protein level).

蛋白家族:

The protein kinase domain mediates binding to phosphatidylinositol.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

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