产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8188, RRID:AB_2840250.
展开/折叠
90 kDa ribosomal protein S6 kinase 2; HU 2; KS6A2_HUMAN; MAP kinase activated protein kinase 1c; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; MAPKAPK1C; Mitogen.activated protein kinase-activated protein kinase 1C; p90 RSK3; p90-RSK 2; p90RSK2; pp90RSK3; Ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; Ribosomal S6 kinase 3; RPS6KA2; RSK 3; RSK; RSK-3; S6K alpha; S6K alpha 2; S6K-alpha-2;
抗原和靶标
Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
- Q15349 KS6A2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLSMKKFAVRRFFSVYLRRKSRSKSSSLSRLEEEGVVKEIDISHHVKEGFEKADPSQFELLKVLGQGSYGKVFLVRKVKGSDAGQLYAMKVLKKATLKVRDRVRSKMERDILAEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALALDHLHSLGIIYRDLKPENILLDEEGHIKITDFGLSKEAIDHDKRAYSFCGTIEYMAPEVVNRRGHTQSADWWSFGVLMFEMLTGSLPFQGKDRKETMALILKAKLGMPQFLSGEAQSLLRALFKRNPCNRLGAGIDGVEEIKRHPFFVTIDWNTLYRKEIKPPFKPAVGRPEDTFHFDPEFTARTPTDSPGVPPSANAHHLFRGFSFVASSLIQEPSQQDLHKVPVHPIVQQLHGNNIHFTDGYEIKEDIGVGSYSVCKRCVHKATDTEYAVKIIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKFVYLVMELMRGGELLDRILRQRYFSEREASDVLCTITKTMDYLHSQGVVHRDLKPSNILYRDESGSPESIRVCDFGFAKQLRAGNGLLMTPCYTANFVAPEVLKRQGYDAACDIWSLGILLYTMLAGFTPFANGPDDTPEEILARIGSGKYALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPWVVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQRRGMKRLTSTRL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15349 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S26 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| K91 | Acetylation | Uniprot | |
| K94 | Acetylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| Y135 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | O15530 (PDPK1) , Q15349 (RPS6KA2) | Uniprot |
| T222 | Phosphorylation | Uniprot | |
| K295 | Acetylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| Y426 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| Y463 | Phosphorylation | Uniprot | |
| T471 | Phosphorylation | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| T570 | Phosphorylation | Q15349 (RPS6KA2) | Uniprot |
| Y573 | Phosphorylation | Uniprot | |
| T574 | Phosphorylation | Uniprot | |
| T618 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| T662 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Uniprot |
翻译修饰 - Q15349 作为激酶
研究背景
Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
Activated by phosphorylation at Ser-218 by PDPK1. Autophosphorylated on Ser-377, as part of the activation process. May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 and MAPK3/ERK1 (By similarity).
N-terminal myristoylation results in an activated kinase in the absence of added growth factors.
Nucleus. Cytoplasm.
Widely expressed with higher expression in lung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.
Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity). Interacts with FBXO5; cooperate to induce the metaphase arrest of early blastomeres; increases and stabilizes interaction of FBXO5 with CDC20 (By similarity).
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
研究领域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
限制条款
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