Phospho-eIF2B epsilon (Ser540) Antibody - #AF8203

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产品: 磷酸化 eIF2B epsilon (Ser540) 抗体
货号: AF8203
描述: Rabbit polyclonal antibody to Phospho-eIF2B epsilon (Ser540)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Chicken
分子量: 80KD; 80kD(Calculated).
蛋白号: Q13144
RRID: AB_2840265

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(80%), Bovine(80%), Horse(80%), Rabbit(80%), Dog(80%), Chicken(80%)
克隆:
Polyclonal
特异性:
Phospho-eIF2B epsilon (Ser540) Antibody detects endogenous levels of eIF2B epsilon only when phosphorylated at Ser540.
RRID:
AB_2840265
引用格式: Affinity Biosciences Cat# AF8203, RRID:AB_2840265.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CACH; CLE; EI2BE_HUMAN; EIF 2B; eIF 2B GDP GTP exchange factor; eIF 2B GDP GTP exchange factor subunit epsilon; eIF-2B GDP-GTP exchange factor subunit epsilon; EIF2B; EIF2B5; EIF2BE; EIF2Bepsilon; Eukaryotic Translation Initiation Factor 2 Beta Epsilon; Eukaryotic translation initiation factor 2B subunit 5 epsilon 82kDa; Eukaryotic translation initiation factor 2B subunit 5 epsilon; LVWM; Translation initiation factor eIF 2B subunit epsilon; Translation initiation factor eIF-2B subunit epsilon;

抗原和靶标

免疫原:
Uniprot:

Q13144(EI2BE_HUMAN) >>Visit HPA database.

基因/基因ID:
EIF2B5(8893)
序列:
MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAAQIKEHLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDVISNINITRALEEHRLRRKLEKNVSVMTMIFKESSPSHPTRCHEDNVVVAVDSTTNRVLHFQKTQGLRRFAFPLSLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQTRDDFVRGLLVNEEILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTDSTTQSCTHSRHNIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNVVLDQTYLWQGVRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVISLHPPDAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESSEDD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
80
Horse
80
Bovine
80
Dog
80
Chicken
80
Rabbit
80
Sheep
70
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13144 作为底物

Site PTM Type Enzyme
A2 Acetylation
S20 Phosphorylation
S27 Phosphorylation
S51 Phosphorylation P48729 (CSNK1A1)
K61 Ubiquitination
K103 Ubiquitination
K108 Ubiquitination
K141 Ubiquitination
T182 Phosphorylation
K186 Ubiquitination
K217 Ubiquitination
Y319 Phosphorylation
K420 Ubiquitination
S447 Phosphorylation
S450 Phosphorylation
S466 Phosphorylation P48729 (CSNK1A1)
S469 Phosphorylation P48729 (CSNK1A1)
K481 Ubiquitination
Y483 Phosphorylation
K493 Ubiquitination
S525 Phosphorylation
S527 Phosphorylation
S529 Phosphorylation
S532 Phosphorylation
S535 Phosphorylation P49841 (GSK3B)
S540 Phosphorylation P49841 (GSK3B) , P49840 (GSK3A)
S544 Phosphorylation P49840 (GSK3A) , P49841 (GSK3B)
K565 Ubiquitination
Y583 Phosphorylation
S610 Phosphorylation
C618 S-Nitrosylation
K693 Ubiquitination
S717 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S718 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)

研究背景

功能:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

翻译修饰:

Phosphorylated at Ser-544 by DYRK2; this is required for subsequent phosphorylation by GSK3B (By similarity). Phosphorylated on serine and threonine residues by GSK3B; phosphorylation inhibits its function.

Polyubiquitinated, probably by NEDD4.

亚基结构:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon. Interacts with RGS2.

蛋白家族:

Belongs to the eIF-2B gamma/epsilon subunits family.

研究领域

· Genetic Information Processing > Translation > RNA transport.

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