产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8243, RRID:AB_2840305.
展开/折叠
BAG 3; BAG family molecular chaperone regulator 3; BAG-3; Bag3; BAG3_HUMAN; Bcl 2 binding protein; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; BCL2 associated athanogene 3; BCL2 binding athanogene 3; BIS; CAIR 1; Docking protein CAIR 1; Docking protein CAIR-1; MFM6;
抗原和靶标
- O95817 BAG3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAATHSPMMQVASGNGDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPSEGPKETPSSANGPSREGSRLPPAREGHPVYPQLRPGYIPIPVLHEGAENRQVHPFHVYPQPGMQRFRTEAAAAAPQRSQSPLRGMPETTQPDKQCGQVAAAAAAQPPASHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYISIPVIHEQNVTRPAAQPSFHQAQKTHYPAQQGEYQTHQPVYHKIQGDDWEPRPLRAASPFRSSVQGASSREGSPARSSTPLHSPSPIRVHTVVDRPQQPMTHRETAPVSQPENKPESKPGPVGPELPPGHIPIQVIRKEVDSKPVSQKPPPPSEKVEVKVPPAPVPCPPPSPGPSAVPSSPKSVATEERAAPSTAPAEATPPKPGEAEAPPKHPGVLKVEAILEKVQGLEQAVDNFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEADQPLQAIMEMGAVAADKGKKNAGNAEDPHTETQQPEATAAATSNPSSMTDTPGNPAAP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95817 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| K29 | Acetylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| R90 | Methylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| R106 | Methylation | Uniprot | |
| Y114 | Phosphorylation | Uniprot | |
| R121 | Methylation | Uniprot | |
| R123 | Methylation | Uniprot | |
| R133 | Methylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| R139 | Methylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| C151 | S-Nitrosylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| C179 | S-Nitrosylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| S190 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| R203 | Methylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| R218 | Methylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K230 | Methylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| Y240 | Phosphorylation | Uniprot | |
| T242 | Phosphorylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| R258 | Methylation | Uniprot | |
| R261 | Methylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| R267 | Methylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S289 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S291 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| K445 | Acetylation | Uniprot | |
| K445 | Sumoylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| Y451 | Phosphorylation | Uniprot | |
| Y457 | Phosphorylation | Uniprot | |
| T459 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| Y508 | Phosphorylation | Uniprot |
研究背景
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport.
Nucleus. Cytoplasm.
Note: Colocalizes with HSF1 to the nucleus upon heat stress (PubMed:26159920).
Binds to the ATPase domain of HSP70/HSC70 chaperones. Interacts with BCL2. Interacts with phospholipase C-gamma proteins. Interacts with DNAJB6. Interacts (via BAG domain) with HSF1; this interaction occurs in normal and heat-shocked cells promoting HSF1 nucleocytoplasmic shuttling. Interacts with HSPA8 (via NBD). Interacts with HSPA1A (via NBD) and HSPA1B (via NBD). Interacts (via WW domain 1) with SYNPO2 (via PPPY motif). Interacts with HSPB8.
限制条款
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