Phospho-Calpain 2 (Ser50) Antibody - #AF8251
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8251, RRID:AB_2840313.
展开/折叠
Calcium activated neutral proteinase 2; Calcium activated neutral proteinase; Calcium-activated neutral protease 2, catalytic subunit; Calcium-activated neutral proteinase 2; CALP80; Calpain 2 (m/II) large subunit; Calpain 2 catalytic subunit; Calpain 2 large catalytic subunit; Calpain 2 large subunit; Calpain 2, large [catalytic] subunit; Calpain large polypeptide L2; Calpain M type; Calpain M-type; Calpain-2 catalytic subunit; Calpain-2 large subunit; Calpain2; CAN2_HUMAN; CANP 2; CANP L2; CANP2; CANPL 2; CANPL2; CANPml; Capa2; CAPN 2; CAPN2; FLJ39928; M calpain; M calpin; M type; M-calpain; mCANP; Millimolar calpain; Millimolar-calpain;
抗原和靶标
- P17655 CAN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGIAAKLAKDREAAEGLGSHDRAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P17655 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K7 | Acetylation | Uniprot | |
| R23 | Methylation | Uniprot | |
| K26 | Ubiquitination | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| K61 | Acetylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| C82 | S-Nitrosylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| K354 | Acetylation | Uniprot | |
| K355 | Acetylation | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| S369 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T370 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K390 | Sumoylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| C577 | S-Nitrosylation | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| S589 | Phosphorylation | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| Y611 | Phosphorylation | Uniprot | |
| T621 | Phosphorylation | Uniprot | |
| Y625 | Phosphorylation | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| K674 | Ubiquitination | Uniprot |
研究背景
Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).
Cytoplasm. Cell membrane.
Note: Translocates to the plasma membrane upon Ca(2+) binding.
Ubiquitous.
Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Interacts with CPEB3; this leads to cleavage of CPEB3.
Belongs to the peptidase C2 family.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
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