| 产品: | 磷酸化 CdGAP (Thr789) 抗体 |
| 货号: | AF8252 |
| 描述: | Rabbit polyclonal antibody to Phospho-CdGAP (Thr789) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Sheep, Rabbit, Dog |
| 分子量: | 157KD; 157kD(Calculated). |
| 蛋白号: | Q2M1Z3 |
| RRID: | AB_2840314 |
产品描述
来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-CdGAP (Thr789) Antibody detects endogenous levels of CdGAP only when phosphorylated at Thr789.
RRID:
AB_2840314
引用格式: Affinity Biosciences Cat# AF8252, RRID:AB_2840314.
引用格式: Affinity Biosciences Cat# AF8252, RRID:AB_2840314.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
ARHGAP31; Cdc42 GTPase-activating protein; RHG31_HUMAN; Rho GTPase-activating protein 31;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
序列:
- Q2M1Z3 RHG31_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKNKGAKQKLKRKGAASAFGCDLTEYLESSGQDVPYVLKSCAEFIETHGIVDGIYRLSGVTSNIQRLRQEFGSDQCPDLTREVYLQDIHCVGSLCKLYFRELPNPLLTYELYEKFTEAVSHCPEEGQLARIQNVIQELPPSHYRTLEYLIRHLAHIASFSSKTNMHARNLALVWAPNLLRSKEIEATGCNGDAAFLAVRVQQVVIEFILNHVDQIFNNGAPGSLENDENRPIMKSLTLPALSLPMKLVSLEEAQARSLATNHPARKERRENSLPEIVPPMGTLFHTVLELPDNKRKLSSKSKKWKSIFNLGRSGSDSKSKLSRNGSVFVRGQRLSVEKATIRPAKSMDSLCSVPVEGKETKGNFNRTVTTGGFFIPATKMHSTGTGSSCDLTKQEGEWGQEGMPPGAEGGFDVSSDRSHLQGAQARPPPEQLKVFRPVEDPESEQTAPKMLGMFYTSNDSPSKSVFTSSLFQMEPSPRNQRKALNISEPFAVSVPLRVSAVISTNSTPCRTPPKELQSLSSLEEFSFHGSESGGWPEEEKPLGAETSAASVPKKAGLEDAKAVPEAPGTVECSKGLSQEPGAHLEEKKTPESSLSSQHLNELEKRPNPEKVVEEGREAGEMESSTLQESPRARAEAVLLHEMDEDDLANALIWPEIQQELKIIESEEELSSLPPPALKTSPIQPILESSLGPFIPSEPPGSLPCGSFPAPVSTPLEVWTRDPANQSTQGASTAASREKPEPEQGLHPDLASLAPLEIVPFEKASPQATVEVGGPGNLSPPLPPAPPPPTPLEESTPVLLSKGGPEREDSSRKLRTDLYIDQLKSQDSPEISSLCQGEEATPRHSDKQNSKNAASEGKGCGFPSPTREVEIVSQEEEDVTHSVQEPSDCDEDDTVTDIAQHGLEMVEPWEEPQWVTSPLHSPTLKDAHKAQVQGLQGHQLEKRLSHRPSLRQSHSLDSKPTVKSQWTLEVPSSSSCANLETERNSDPLQPQAPRREITGWDEKALRSFREFSGLKGAEAPPNQKGPSGVQPNPAETSPISLAEGKELGTHLGHSSPQIRQGGVPGPESSKESSPSVQDSTSPGEHPAKLQLKSTECGPPKGKNRPSSLNLDPAIPIADLFWFENVASFSSPGMQVSEPGDPKVTWMTSSYCKADPWRVYSQDPQDLDIVAHALTGRRNSAPVSVSAVRTSFMVKMCQARAVPVIPPKIQYTQIPQPLPSQSSGENGVQPLERSQEGPSSTSGTTQKPAKDDSPSSLESSKEEKPKQDPGAIKSSPVDATAPCMCEGPTLSPEPGSSNLLSTQDAVVQCRKRMSETEPSGDNLLSSKLERPSGGSKPFHRSRPGRPQSLILFSPPFPIMDHLPPSSTVTDSKVLLSPIRSPTQTVSPGLLCGELAENTWVTPEGVTLRNKMTIPKNGQRLETSTSCFYQPQRRSVILDGRSGRQIE
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q2M1Z3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T237 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| T340 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T511 | Phosphorylation | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S778 | Phosphorylation | Uniprot | |
| T789 | Phosphorylation | P49840 (GSK3A) , P27361 (MAPK3) | Uniprot |
| S827 | Phosphorylation | Uniprot | |
| T840 | Phosphorylation | Uniprot | |
| S844 | Phosphorylation | Uniprot | |
| S849 | Phosphorylation | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| S920 | Phosphorylation | Uniprot | |
| S944 | Phosphorylation | Uniprot | |
| S948 | Phosphorylation | Uniprot | |
| S954 | Phosphorylation | Uniprot | |
| K1002 | Methylation | Uniprot | |
| S1036 | Phosphorylation | Uniprot | |
| S1053 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | Uniprot | |
| S1106 | Phosphorylation | Uniprot | |
| S1178 | Phosphorylation | Uniprot | |
| S1182 | Phosphorylation | Uniprot | |
| S1184 | Phosphorylation | Uniprot | |
| S1312 | Phosphorylation | Uniprot | |
| S1317 | Phosphorylation | Uniprot | |
| S1339 | Phosphorylation | Uniprot | |
| S1374 | Phosphorylation | Uniprot | |
| S1432 | Phosphorylation | Uniprot |
研究背景
功能:
Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.
翻译修饰:
Phosphorylation on Thr-789 reduces GAP activity.
细胞定位:
Cell projection>Lamellipodium. Cell junction>Focal adhesion.
亚基结构:
Interacts with ITSN1, which inhibits GAP activity. Interacts with PARVA (By similarity). Interacts with GTP-loaded RHOU.
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