产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8255, RRID:AB_2840317.
展开/折叠
Claspin; claspin homolog (Xenopus laevis); Claspin homolog; CLSPN; CLSPN protein; CLSPN_HUMAN; hClaspin; Hu Claspin;
抗原和靶标
- Q9HAW4 CLSPN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTGEVGSEVHLEINDPNVISQEEADSPSDSGQGSYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSETEDTNASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSLYQENLEAQVKPCLELSLQSGNSTDFTTDRKSSKKHIHDKEGTAGKAKVKSKRRLEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLVDKDLFETGLEDENNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSKGTTRKERKAARLSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFKRKPRPTCHGNAMALLKSSKYQSSHHKEIIDTANTTEMNSDHHSKGSEQTTGAENEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIRPSPGDSSVLQQESNFLGNNHSEECQVGGLVAFEPHALEGEGPQNPEETDEKVEEPEQQNKSSAVGPPEKVRRFTLDRLKQLGVDVSIKPRLGADEDSFVILEPETNRELEALKQRFWKHANPAAKPRAGQTVNVNVIVKDMGTDGKEELKADVVPVTLAPKKLDGASHTKPGEKLQVLKAKLQEAMKLRRFEERQKRQALFKLDNEDGFEEEEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEEEEEGNQETAEFLLSSEEIETKDEKEMDKENNDGSSEIGKAVGFLSVPKSLSSDSTLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCSLLTKESSHNSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLVSSASKSSGKLSEPSLPIEDSQDLYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVRNHRNQYQALKPRLPLASMDENAMDANMDELLDLCTGKFTSQAEKHLPRKSDKKENMEELLNLCSGKFTSQDASTPASSELNKQEKESSMGDPMEEALALCSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSDSGNDLALEDHEDDDEEELLKRSEKLKRQMRLRKYLEDEAEVSGSDVGSEDEYDGEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQLRLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESEARWRKERIEREQWLRDMAQQGKITAEEEEEIGEDSQFMILAKKVTAKALQKNASRPMVIQESKSLLRNPFEAIRPGSAQQVKTGSLLNQPKAVLQKLAALSDHNPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKHLKTDDSTSGLTRSIFKYLES
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9HAW4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S30 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S34 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | O00311 (CDC7) | Uniprot |
| T69 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| Y81 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | O00311 (CDC7) | Uniprot |
| Y120 | Phosphorylation | Uniprot | |
| K187 | Acetylation | Uniprot | |
| K188 | Acetylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S265 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S282 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| Y306 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K403 | Sumoylation | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| K606 | Acetylation | Uniprot | |
| T639 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S703 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | O00311 (CDC7) | Uniprot |
| K708 | Ubiquitination | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| S720 | Phosphorylation | Uniprot | |
| S721 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S723 | Phosphorylation | Uniprot | |
| T724 | Phosphorylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| S744 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S750 | Phosphorylation | Uniprot | |
| K756 | Acetylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| S774 | Phosphorylation | Uniprot | |
| S775 | Phosphorylation | Uniprot | |
| T802 | Phosphorylation | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| S823 | Phosphorylation | Uniprot | |
| K824 | Ubiquitination | Uniprot | |
| K828 | Ubiquitination | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| S839 | Phosphorylation | Uniprot | |
| Y843 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| Y887 | Phosphorylation | Uniprot | |
| K891 | Acetylation | Uniprot | |
| K891 | Ubiquitination | Uniprot | |
| T916 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S921 | Phosphorylation | Uniprot | |
| K925 | Acetylation | Uniprot | |
| K925 | Ubiquitination | Uniprot | |
| S945 | Phosphorylation | Uniprot | |
| K947 | Ubiquitination | Uniprot | |
| T949 | Phosphorylation | Uniprot | |
| S950 | Phosphorylation | Uniprot | |
| S954 | Phosphorylation | Uniprot | |
| T955 | Phosphorylation | Uniprot | |
| S958 | Phosphorylation | Uniprot | |
| S959 | Phosphorylation | Uniprot | |
| K963 | Ubiquitination | Uniprot | |
| S982 | Phosphorylation | Uniprot | |
| S984 | Phosphorylation | Q9BTU6 (PI4K2A) | Uniprot |
| T987 | Phosphorylation | Uniprot | |
| S1012 | Phosphorylation | Uniprot | |
| S1018 | Phosphorylation | Uniprot | |
| S1020 | Phosphorylation | Uniprot | |
| S1129 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S1147 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S1156 | Phosphorylation | Uniprot | |
| T1161 | Phosphorylation | Uniprot | |
| S1204 | Phosphorylation | Uniprot | |
| S1223 | Phosphorylation | Uniprot | |
| K1265 | Ubiquitination | Uniprot | |
| T1287 | Phosphorylation | Uniprot | |
| S1289 | Phosphorylation | Uniprot | |
| K1292 | Sumoylation | Uniprot | |
| S1310 | Phosphorylation | Uniprot | |
| S1314 | Phosphorylation | Uniprot | |
| S1316 | Phosphorylation | Uniprot | |
| K1321 | Sumoylation | Uniprot | |
| K1321 | Ubiquitination | Uniprot | |
| T1322 | Phosphorylation | Uniprot |
研究背景
Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.
Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.
Nucleus.
Interacts (phosphorylation-dependent) with CHEK1; regulates CLSPN function in checkpoint for DNA damage and replication. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Interacts with TIMELESS.
The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.
Belongs to the claspin family.
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