Phospho-CtBP1/2 (Ser158/Ser164) Antibody - #AF8259

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产品: 磷酸化 CtBP1/2 (Ser158/Ser164) 抗体
货号: AF8259
描述: Rabbit polyclonal antibody to Phospho-CtBP1/2 (Ser158/Ser164)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Dog, Chicken, Xenopus
分子量: 48KD; 48kD,49kD(Calculated).
蛋白号: Q13363 | P56545
RRID: AB_2840321

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 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-CtBP1/2 (Ser158/Ser164) Antibody detects endogenous levels of CtBP1/2 only when phosphorylated at Ser158/164.
RRID:
AB_2840321
引用格式: Affinity Biosciences Cat# AF8259, RRID:AB_2840321.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BARS; brefeldin A- ribosylated substrate; C terminal binding protein 1; C-terminal-binding protein 1; CTBP; CtBP1; CTBP1_HUMAN; MGC104684; C terminal binding protein 2; C-terminal-binding protein 2; CtBP2; CTBP2_HUMAN; ribeye;

抗原和靶标

免疫原:
Uniprot:

Q13363(CTBP1_HUMAN) >>Visit HPA database.

P56545(CTBP2_HUMAN) >>Visit HPA database.

基因/基因ID:
CTBP2(1488) | CTBP1(1487)
表达:
Q13363 CTBP1_HUMAN:

Expressed in germinal center B-cells.

P56545 CTBP2_HUMAN:

Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

序列:
MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL

MALVDKHKVKRQRLDRICEGIRPQIMNGPLHPRPLVALLDGRDCTVEMPILKDLATVAFCDAQSTQEIHEKVLNEAVGAMMYHTITLTREDLEKFKALRVIVRIGSGYDNVDIKAAGELGIAVCNIPSAAVEETADSTICHILNLYRRNTWLYQALREGTRVQSVEQIREVASGAARIRGETLGLIGFGRTGQAVAVRAKAFGFSVIFYDPYLQDGIERSLGVQRVYTLQDLLYQSDCVSLHCNLNEHNHHLINDFTIKQMRQGAFLVNAARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFAQGPLKDAPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIPESLRNCVNKEFFVTSAPWSVIDQQAIHPELNGATYRYPPGIVGVAPGGLPAAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
82
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13363/P56545 作为底物

Site PTM Type Enzyme
K46 Ubiquitination
C54 S-Nitrosylation
Y76 Phosphorylation
K88 Ubiquitination
S100 Phosphorylation
T144 Phosphorylation
S158 Phosphorylation Q13153 (PAK1) , Q13131 (PRKAA1)
K194 Ubiquitination
R266 Methylation
K273 Ubiquitination
K280 Ubiquitination
R285 Methylation
S300 Phosphorylation
K348 Ubiquitination
C350 S-Nitrosylation
S422 Phosphorylation P45983 (MAPK8) , Q9H2X6 (HIPK2)
K428 Sumoylation
Site PTM Type Enzyme
K6 Acetylation
K8 Acetylation
K10 Acetylation
R22 Methylation
R33 Methylation
K52 Ubiquitination
K94 Ubiquitination
Y108 Phosphorylation
S164 Phosphorylation
K279 Ubiquitination
K286 Ubiquitination
R291 Methylation
S365 Phosphorylation
S369 Phosphorylation
T414 Phosphorylation
S428 Phosphorylation Q9H2X6 (HIPK2)

研究背景

功能:

Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.

翻译修饰:

The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.

ADP-ribosylated; when cells are exposed to brefeldin A.

Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in germinal center B-cells.

亚基结构:

Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)-specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZFHX1B and WIZ. Interacts with ZNF366 (via PXDLS motif). Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Interacts with BCL6; the interaction is required for BCL6 transcriptional autoinhibition and inhibition of some BCL6 target genes. Interacts with IKZF4 (By similarity). Interacts with MCRIP1 (unphosphorylated form, via the PXDLS motif); competitively inhibiting CTBP-ZEB1 interaction. Interacts with Bassoon/BSN; this interaction targets and anchors CTBP1 to presynapses (By similarity).

(Microbial infection) Interacts with Epstein-Barr virus EBNA3 and EBNA6.

(Microbial infection) Interacts with adenovirus E1A protein (via its C-terminus); the interaction disrupts the interaction of CTBP1 with RBBP8.

(Microbial infection) Interacts with human adenovirus 5 E1A protein; this interaction seems to potentiate viral replication.

蛋白家族:

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

功能:

Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity).

Isoform 2 probably acts as a scaffold for specialized synapses.

细胞定位:

Nucleus. Cell junction>Synapse.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

亚基结构:

Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with HIPK2 and ZNF217. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression (By similarity). Interacts with PNN, NRIP1 and WIZ. Interacts with MCRIP1.

(Microbial infection) Interacts with human adenovirus 5 E1A protein; this interaction seems to potentiate viral replication.

蛋白家族:

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

研究领域

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

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