Phospho-Connexin 43 / GJA1 (Ser373) Antibody - #AF8264

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产品: 磷酸化 Connexin 43 / GJA1 (Ser373) 抗体
货号: AF8264
描述: Rabbit polyclonal antibody to Phospho-Connexin 43 / GJA1 (Ser373)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 43kDa; 43kD(Calculated).
蛋白号: P17302
RRID: AB_2840326

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-Connexin 43 / GJA1 (Ser373) Antibody detects endogenous levels of Connexin 43 / GJA1 only when phosphorylated at Ser373.
RRID:
AB_2840326
引用格式: Affinity Biosciences Cat# AF8264, RRID:AB_2840326.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Connexin 43; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1; GJAL; ODD; ODDD; ODOD; SDTY3;

抗原和靶标

免疫原:
Uniprot:

P17302(CXA1_HUMAN) >>Visit HPA database.

基因/基因ID:
GJA1(2697)
表达:
P17302 CXA1_HUMAN:

Expressed in the heart and fetal cochlea.

序列:
MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P17302 作为底物

Site PTM Type Enzyme
K9 Ubiquitination
K109 Ubiquitination
K128 Ubiquitination
K136 Ubiquitination
Y137 Phosphorylation
K144 Ubiquitination
R148 Methylation
K243 Ubiquitination
S244 Phosphorylation
Y247 Phosphorylation P12931 (SRC)
T250 Phosphorylation
S251 Phosphorylation
S255 Phosphorylation P28482 (MAPK1) , P06493 (CDK1) , P27361 (MAPK3) , Q13164 (MAPK7)
K258 Ubiquitination
S262 Phosphorylation P17252 (PRKCA)
K264 Ubiquitination
Y265 Phosphorylation P12931 (SRC)
Y267 Phosphorylation
S272 Phosphorylation
T275 Phosphorylation
S279 Phosphorylation Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3)
S282 Phosphorylation P28482 (MAPK1) , Q16539 (MAPK14) , P27361 (MAPK3) , Q13164 (MAPK7)
Y286 Phosphorylation
K287 Ubiquitination
T290 Phosphorylation
S296 Phosphorylation
S297 Phosphorylation
Y301 Phosphorylation
K303 Ubiquitination
S306 Phosphorylation
Y313 Phosphorylation
S314 Phosphorylation
S325 Phosphorylation P48730 (CSNK1D)
T326 Phosphorylation
S328 Phosphorylation P48730 (CSNK1D)
S330 Phosphorylation P48730 (CSNK1D)
S344 Phosphorylation
K345 Ubiquitination
K346 Ubiquitination
S364 Phosphorylation
S365 Phosphorylation Q02156 (PRKCE) , P17612 (PRKACA)
S368 Phosphorylation P17252 (PRKCA) , P17612 (PRKACA) , Q02156 (PRKCE) , P05771 (PRKCB) , P05129 (PRKCG) , Q05655 (PRKCD)
S369 Phosphorylation P31749 (AKT1) , Q02156 (PRKCE) , P17612 (PRKACA)
S372 Phosphorylation
S373 Phosphorylation P17612 (PRKACA) , P31749 (AKT1) , Q02156 (PRKCE)

研究背景

功能:

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).

翻译修饰:

Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity).

Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2.

S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.

Acetylated in the developing cortex; leading to delocalization from the cell membrane.

细胞定位:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction. Endoplasmic reticulum.
Note: Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the heart and fetal cochlea.

亚基结构:

A connexon is composed of a hexamer of connexins. Interacts (via C-terminus) with TJP1 (By similarity). Interacts (via C-terminus) with SRC (via SH3 domain) (By similarity). Interacts (not ubiquitinated) with UBQLN4 (via UBA domain) (By similarity). Interacts with SGSM3 and CNST (By similarity). Interacts with RIC1/CIP150. Interacts with CSNK1D. Interacts with NOV. Interacts with TMEM65 (By similarity).

蛋白家族:

Belongs to the connexin family. Alpha-type (group II) subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

文献引用

1). Rat Hepatocytes Mitigate Cadmium Toxicity by Forming Annular Gap Junctions and Degrading Them via Endosome–Lysosome Pathway. International Journal of Molecular Sciences, 2022 (PubMed: 36555247) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 1 Cd induces Cx43 internalization and degradation in hepatocytes: (A) Cx43 was co-stained with microfilaments (labeled with phalloidin). Green represents microfilaments, and red represents Cx43. The white rectangle represents Cx43 between cells under different Cd concentrations. The white arrow represents GJP. Scale bar = 10 μm; (B) Anti-Cx43 antibody was co-stained with anti-α-tubulin-FITC antibody. Green represents maicrotubules, and red represents Cx43. The white rectangle represents Cx43 between cells under different Cd concentrations. The white arrow represents GJP. Scale bar = 10 μm; (C,D) Western blotting analysis of the Cx43 level in rat hepatocytes. Statistical results were obtained from at least three independent experiments; * p < 0.05 compared to the control group, ** p < 0.01 compared to the control group; (E,F) Western blotting analysis of P-Cx43 (Ser 373) level; ** p < 0.01 compared to the control group, ## p < 0.01 compared to the Cd group.

Application: IF/ICC    Species: Rat    Sample:

Figure 1 Cd induces Cx43 internalization and degradation in hepatocytes: (A) Cx43 was co-stained with microfilaments (labeled with phalloidin). Green represents microfilaments, and red represents Cx43. The white rectangle represents Cx43 between cells under different Cd concentrations. The white arrow represents GJP. Scale bar = 10 μm; (B) Anti-Cx43 antibody was co-stained with anti-α-tubulin-FITC antibody. Green represents maicrotubules, and red represents Cx43. The white rectangle represents Cx43 between cells under different Cd concentrations. The white arrow represents GJP. Scale bar = 10 μm; (C,D) Western blotting analysis of the Cx43 level in rat hepatocytes. Statistical results were obtained from at least three independent experiments; * p < 0.05 compared to the control group, ** p < 0.01 compared to the control group; (E,F) Western blotting analysis of P-Cx43 (Ser 373) level; ** p < 0.01 compared to the control group, ## p < 0.01 compared to the Cd group.

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