Phospho-Calcipressin 1 (Ser163) Antibody - #AF8268

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产品: 磷酸化 Calcipressin 1 (Ser163) 抗体
货号: AF8268
描述: Rabbit polyclonal antibody to Phospho-Calcipressin 1 (Ser163)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 22 KD; 28kD(Calculated).
蛋白号: P53805
RRID: AB_2840330

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(86%)
克隆:
Polyclonal
特异性:
Phospho-Calcipressin 1 (Ser163) Antibody detects endogenous levels of Calcipressin 1 only when phosphorylated at Ser163.
RRID:
AB_2840330
引用格式: Affinity Biosciences Cat# AF8268, RRID:AB_2840330.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Adapt 78; Adapt78; Calcipressin 1; Calcipressin-1; Calcium and oxidant inducible mRNA; CSP 1; CSP1; Down syndrome candidate region 1; Down syndrome critical region gene 1; Down syndrome critical region protein 1; DSC 1; DSC1; DSCR 1; DSCR1; MCIP 1; MCIP1; Modulatory calcineurin interacting protein 1; Myocyte enriched calcineurin interacting protein 1; Myocyte-enriched calcineurin-interacting protein 1; Near DSCR proline rich protein; RCAN1; RCAN1_HUMAN; RCN 1; RCN1; Regulator of calcineurin 1;

抗原和靶标

免疫原:
Uniprot:

P53805(RCAN1_HUMAN) >>Visit HPA database.

基因/基因ID:
RCAN1(1827)
表达:
P53805 RCAN1_HUMAN:

Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues.

序列:
MEDGVAGPQLGAAAEAAEAAEARARPGVTLRPFAPLSGAAEADEGGGDWSFIDCEMEEVDLQDLPSATIACHLDPRVFVDGLCRAKFESLFRTYDKDITFQYFKSFKRVRINFSNPFSAADARLQLHKTEFLGKEMKLYFAQTLHIGSSHLAPPNPDKQFLISPPASPPVGWKQVEDATPVINYDLLYAISKLGPGEKYELHAATDTTPSVVVHVCESDQEKEEEEEMERMRRPKPKIIQTRRPEYTPIHLS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
86
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P53805 作为底物

Site PTM Type Enzyme
K86 Ubiquitination
Y94 Phosphorylation
K128 Ubiquitination
T129 Phosphorylation
K134 Ubiquitination
S163 Phosphorylation Q99759 (MAP3K3) , P49841 (GSK3B)
S167 Phosphorylation Q99759 (MAP3K3) , Q13627 (DYRK1A) , P27361 (MAPK3) , P28482 (MAPK1)
T179 Phosphorylation
Y188 Phosphorylation
K192 Ubiquitination
T208 Phosphorylation
S218 Phosphorylation
T241 Phosphorylation
T247 Phosphorylation Q13627 (DYRK1A)

研究背景

功能:

Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development (By similarity).

翻译修饰:

Phosphorylation increases its ability to inhibit calcineurin and decreases protein half-life.

组织特异性:

Highly expressed heart, brain and skeletal muscle. Also expressed in all other tissues.

亚基结构:

Interacts with RAF1. Interacts with PPP3CA and PPP3R1.

蛋白家族:

Belongs to the RCAN family.

研究领域

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

文献引用

1). Mangiferin Inhibits PDGF-BB-Induced Proliferation and Migration of Rat Vascular Smooth Muscle Cells and Alleviates Neointimal Formation in Mice through the AMPK/Drp1 Axis. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34900084)

Application: WB    Species: Mouse    Sample:

Figure 4 Mangiferin alleviated PDGF-BB-induced mitochondrial fission and increase in ROS levels. (a) RSMCs were stained with mitotracker probe after treatment with different concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. The average length of mitochondria was quantified using ImageJ software. (b) Intracellular ROS levels were detected using flow cytometry after RSMCs were treated with mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. (c–e) Western blot was used to measure the protein expression levels of AMPK, pAMPKThr172, Drp1, and pDrp1Ser616 after RSMCs were treated with various concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. All assays were conducted in triplicate and all data presented as mean ± S.D. ∗P < 0.05, compared with the control group. #P < 0.05, compared with the PDGF-BB group.

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