| 产品: | 磷酸化 Kizuna (Thr379) 抗体 |
| 货号: | AF8277 |
| 描述: | Rabbit polyclonal antibody to Phospho-Kizuna (Thr379) |
| 应用: | WB |
| 反应: | Human |
| 预测: | Rat, Pig, Xenopus |
| 分子量: | 75KD; 75kD(Calculated). |
| 蛋白号: | Q2M2Z5 |
| RRID: | AB_2840339 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8277, RRID:AB_2840339.
展开/折叠
Centrosomal protein kizuna; DKFZP586H021; HT013; Kiz; KIZ_HUMAN; KIZUNA; MGC102941; MGC141930; NCRNA00153; Plk1s1; Polo-like kinase 1 substrate 1; Uncharacterized protein C20orf19;
抗原和靶标
- Q2M2Z5 KIZ_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRTLASAVPLSSPDYYERLGQLQHGLRDSEKKRLDLEKKLYEYNQSDTCRVKLKYVKLKNYLKEICESEKKAHTRNQEYLKRFERVQAHVVHFTTNTEKLQKLKLEYETQIKKMLCSKDSLGLKEELTDEDREKVAVHEGINSGTAMSRGLYQPATIFMGRQMSAILSMRDFSTEHKSPQPTKNFSIPDPHSHRQTAQSSNVTDSCVVQTSNDTQCLNKSDNIDGKASLQIGEKMPVTASVLSEEEQTHCLEIGSNTRHGKSNLSEGKKSAELNSPLRERLSPENRTTDLKCDSSSGSEGEILTREHIEVEEKRASPPVSPIPVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISISEDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPGQTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEAKPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHLSGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNHNTDDSDDFYD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q2M2Z5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K40 | Ubiquitination | Uniprot | |
| Y42 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T379 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S435 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S478 | Phosphorylation | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| T605 | Phosphorylation | Uniprot | |
| S616 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S619 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| T665 | Phosphorylation | Uniprot | |
| S668 | Phosphorylation | Uniprot |
研究背景
Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.
Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.
Interacts with AKAP9, CEP72, ODF2, PCNT and TUBGCP2.
Belongs to the kizuna family.
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