产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8291, RRID:AB_2840353.
展开/折叠
Alpha NAC; Alpha-NAC; Hom s 2.02; HSD48; MGC117224; NAC-alpha; naca; NACA/1.9.2; NACA_HUMAN; NACA1; Nascent polypeptide associated complex alpha polypeptide; Nascent polypeptide associated complex alpha subunit; Nascent polypeptide associated complex subunit alpha; Nascent polypeptide-associated complex subunit alpha; Alpha NAC like; ANAC; Hom s 2.01; MGC71999; NAC alpha like; NACA2; NACAL; nascent polypeptide associated complex alpha polypeptide like; Nascent polypeptide associated complex subunit alpha 2;
抗原和靶标
A synthesized peptide derived from human NACA around the phosphorylation site of Thr159.
Ubiquitously expressed.
Q9H009 NACA2_HUMAN:Expressed specifically in testis and skeletal muscle.
- Q13765 NACA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM
- Q9H009 NACA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGEATETVPATEQELPQSQAETGSGTASDSGESVPGIEEQDSTQTTTQKAWLVAAAEIDEEPVGKAKQSRSEKRARKAMSKLGLLQVTGVTRVTIWKSKNILFVITKLDVYKSPASDAYIVFGEAKIQDLSQQAQLAAAEKFRVQGEAVGNIQENTQTPTVQEESEEEEVDETGVEVKDVKLVMSQANVSRAKAVRALKNNSNDIVNAIMELTV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13765/Q9H009 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S43 | Phosphorylation | Q13418 (ILK) | Uniprot |
| S132 | Phosphorylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| T159 | Phosphorylation | Uniprot | |
| T161 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K68 | Ubiquitination | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| T214 | Phosphorylation | Uniprot |
研究背景
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters.
Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization.
Cytoplasm. Nucleus.
Note: The heterodimer is located mainly in the cytosol, and the homodimer in the nucleus.
Ubiquitously expressed.
Interacts with TBP and JUN (By similarity). Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit and contacts the ribosomal protein L23, which is positioned near the exiting site. Both subunits can contact nascent polypeptide chains. NACA may also form homodimers, and only this form binds DNA.
The positively charged inner surface of the NAC-A/B domain is crucial for NACA localization in the nucleus and DNA-binding. This region is blocked from binding nucleic acids in the heterodimeric complex by a helix region in the beta-subunit, it also displays much higher affinity for RNA than DNA.
Belongs to the NAC-alpha family.
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) (By similarity).
Cytoplasm. Nucleus.
Expressed specifically in testis and skeletal muscle.
Part of the nascent polypeptide-associated complex (NAC), consisting of NACA and BTF3. NAC associates with ribosomes through the BTF3 subunit. Both subunits can contact nascent polypeptide chains (By similarity).
Belongs to the NAC-alpha family.
限制条款
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