| 产品: | 磷酸化 PAK6 (Ser165) 抗体 |
| 货号: | AF8297 |
| 描述: | Rabbit polyclonal antibody to Phospho-PAK6 (Ser165) |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog |
| 分子量: | 75kDa; 75kD(Calculated). |
| 蛋白号: | Q9NQU5 |
| RRID: | AB_2840359 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8297, RRID:AB_2840359.
展开/折叠
CDKN1A activated kinase 6; EC 2.7.1.37; p21 activated protein kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A) activated kinase 6; p21-activated kinase 6; p21activated kinase 6; PAK 5; PAK 6; PAK-5; PAK-6; PAK5; Pak6; PAK6_HUMAN; Serine threonine protein kinase PAK 6; Serine/threonine protein kinase PAK 6; Serine/threonine protein kinase PAK6; Serine/threonine-protein kinase PAK 6;
抗原和靶标
Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.
- Q9NQU5 PAK6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTVVRGSAMPVDGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPTGTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFRSMFLSTAATAPPSSSKPGPPPQSKPNSSFRPPQKDNPPSLVAKAQSLPSDQPVGTFSPLTTSDTSSPQKSLRTAPATGQLPGRSSPAGSPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NQU5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T69 | Phosphorylation | Uniprot | |
| Y81 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| Y129 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| Y144 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| S179 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| T339 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| Y365 | Phosphorylation | Uniprot | |
| T371 | Phosphorylation | Uniprot | |
| K528 | Ubiquitination | Uniprot | |
| S560 | Phosphorylation | Q9NQU5 (PAK6) | Uniprot |
| T564 | Phosphorylation | P52564 (MAP2K6) | Uniprot |
| Y566 | Phosphorylation | P52564 (MAP2K6) | Uniprot |
| S616 | Phosphorylation | Uniprot |
翻译修饰 - Q9NQU5 作为激酶
研究背景
Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.
Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation.
Cytoplasm. Nucleus.
Note: Cotranslocates into nucleus with AR in response to androgen induction.
Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity). Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
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