| 产品: | 磷酸化 PDE4D (Ser715) 抗体 |
| 货号: | AF8298 |
| 描述: | Rabbit polyclonal antibody to Phospho-PDE4D (Ser715) |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 91kDa; 91kD(Calculated). |
| 蛋白号: | Q08499 |
| RRID: | AB_2840360 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8298, RRID:AB_2840360.
展开/折叠
cAMP specific 3',5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D6; cAMP-specific 3''; DPDE3; Dunce like phosphodiesterase E3; FLJ97311; HSPDE4D; PDE43; Pde4d; PDE4D_HUMAN; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila); Phosphodiesterase 4D cAMP specific; Phosphodiesterase 4D, cAMP specific (dunce); STRK1;
抗原和靶标
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
- Q08499 PDE4D_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAEGSSAPARAGSGEGSDSAGGATLKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQPQCPLQPPPPPPLPPPPPPPGAARGRYASSGATGRVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q08499 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S18 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| Y199 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K309 | Methylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K387 | Sumoylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S606 | Phosphorylation | Uniprot | |
| S657 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S746 | Phosphorylation | Uniprot | |
| S761 | Phosphorylation | Uniprot |
研究背景
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.
Apical cell membrane. Cytoplasm. Membrane. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). Colocalized with SHANK2 to the apical membrane of colonic crypt cells.
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind RACK1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Morphine addiction.
· Metabolism > Nucleotide metabolism > Purine metabolism.
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