产品: 磷酸化 PTP1B (Tyr66) 抗体
货号: AF8305
描述: Rabbit polyclonal antibody to Phospho-PTP1B (Tyr66)
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 49kDa; 50kD(Calculated).
蛋白号: P18031
RRID: AB_2840367

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(86%)
克隆:
Polyclonal
特异性:
Phospho-PTP1B (Tyr66) Antibody detects endogenous levels of PTP1B only when phosphorylated at Tyr66.
RRID:
AB_2840367
引用格式: Affinity Biosciences Cat# AF8305, RRID:AB_2840367.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

PTP1B; Non receptor tyrosine phosphatase 1; Protein phosphotyrosylphosphatase 1B; Protein tyrosine phosphatase 1B; Protein tyrosine phosphatase non receptor type 1; Protein tyrosine phosphatase placental; Protein-tyrosine phosphatase 1B; PTN1_HUMAN; PTP 1B; PTP-1B; PTPN 1; PTPN1; Tyrosine protein phosphatase non receptor type 1; Tyrosine-protein phosphatase non-receptor type 1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P18031 PTN1_HUMAN:

Expressed in keratinocytes (at protein level).

序列:
MEMEKEFEQIDKSGSWAAIYQDIRHEASDFPCRVAKLPKNKNRNRYRDVSPFDHSRIKLHQEDNDYINASLIKMEEAQRSYILTQGPLPNTCGHFWEMVWEQKSRGVVMLNRVMEKGSLKCAQYWPQKEEKEMIFEDTNLKLTLISEDIKSYYTVRQLELENLTTQETREILHFHYTTWPDFGVPESPASFLNFLFKVRESGSLSPEHGPVVVHCSAGIGRSGTFCLADTCLLLMDKRKDPSSVDIKKVLLEMRKFRMGLIQTADQLRFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYWKPFLVNMCVATVLTAGAYLCYRFLFNSNT

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
86
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P18031 作为底物

Site PTM Type Enzyme
M1 Acetylation
K5 Ubiquitination
K12 Ubiquitination
S13 Phosphorylation
S15 Phosphorylation
Y20 Phosphorylation
C32 S-Nitrosylation
Y46 Phosphorylation
S50 Phosphorylation P49759 (CLK1) , P31749 (AKT1) , P49760 (CLK2)
S55 Phosphorylation
K58 Ubiquitination
Y66 Phosphorylation P00533 (EGFR) , P06213 (INSR)
S70 Phosphorylation
K73 Ubiquitination
C92 S-Nitrosylation
K116 Ubiquitination
S118 Phosphorylation
K120 Ubiquitination
K128 Ubiquitination
K131 Acetylation
K131 Ubiquitination
K141 Acetylation
S146 Phosphorylation
K150 Ubiquitination
Y152 Phosphorylation P06213 (INSR)
Y153 Phosphorylation P06213 (INSR)
S201 Phosphorylation
S205 Phosphorylation
C215 S-Nitrosylation
S222 Phosphorylation
T224 Phosphorylation
K239 Ubiquitination
S242 Phosphorylation P49759 (CLK1) , P49760 (CLK2)
S243 Phosphorylation P49759 (CLK1) , P49760 (CLK2)
K247 Ubiquitination
S286 Phosphorylation P53350 (PLK1)
S295 Phosphorylation
K314 Ubiquitination
K323 Ubiquitination
K335 Sumoylation
K335 Ubiquitination
T338 Phosphorylation
K347 Sumoylation
K350 Ubiquitination
S352 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
Y359 Phosphorylation
S363 Phosphorylation
S365 Phosphorylation
T368 Phosphorylation
S378 Phosphorylation P68400 (CSNK2A1) , P17252 (PRKCA) , P19784 (CSNK2A2)
R380 Methylation
S386 Phosphorylation P06493 (CDK1) , P68400 (CSNK2A1) , P19784 (CSNK2A2)
S393 Phosphorylation P53350 (PLK1)

研究背景

功能:

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

翻译修饰:

Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.

Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.

S-nitrosylation of Cys-215 inactivates the enzyme activity.

Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.

细胞定位:

Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Interacts with EPHA3 at the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in keratinocytes (at protein level).

亚基结构:

Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts with MET.

蛋白家族:

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.