产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8310, RRID:AB_2840372.
展开/折叠
40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein;
抗原和靶标
- P62753 RS6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P62753 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Methylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| C12 | S-Nitrosylation | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| R22 | Methylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K79 | Acetylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| R154 | Methylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| Y209 | Phosphorylation | Uniprot | |
| K211 | Acetylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K218 | Acetylation | Uniprot | |
| S235 | Phosphorylation | Q13177 (PAK2) , P51812 (RPS6KA3) , P53355 (DAPK1) , Q05655 (PRKCD) , P23443 (RPS6KB1) , Q96RG2 (PASK) , Q15418 (RPS6KA1) | Uniprot |
| S236 | Phosphorylation | Q05655 (PRKCD) , P23443 (RPS6KB1) , P51812 (RPS6KA3) , Q13177 (PAK2) , Q15418 (RPS6KA1) , Q96RG2 (PASK) | Uniprot |
| S240 | Phosphorylation | Q13177 (PAK2) , P23443 (RPS6KB1) , Q15418 (RPS6KA1) | Uniprot |
| T241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| S244 | Phosphorylation | Q15418 (RPS6KA1) , P23443 (RPS6KB1) | Uniprot |
| S246 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | P23443 (RPS6KB1) | Uniprot |
研究背景
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.
Belongs to the eukaryotic ribosomal protein eS6 family.
研究领域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Genetic Information Processing > Translation > Ribosome.
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
限制条款
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