产品: 磷酸化 USF1 (Thr153) 抗体
货号: AF8331
描述: Rabbit polyclonal antibody to Phospho-USF1 (Thr153)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit, Dog
分子量: 34kDa; 34kD(Calculated).
蛋白号: P22415
RRID: AB_2840393

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-USF1 (Thr153) Antibody detects endogenous levels of USF1 only when phosphorylated at Thr153.
RRID:
AB_2840393
引用格式: Affinity Biosciences Cat# AF8331, RRID:AB_2840393.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bHLHb11; Class B basic helix-loop-helix protein 11; FCHL 1; FCHL; FCHL1; HGNC:12593; HYPLIP 1; HYPLIP1; Major late transcription factor 1; Major late transcription factor; MLTF; MLTF I; MLTFI; Transcription factor USF-1; UEF; Upstream stimulatory factor 1; Upstream transcription factor 1; Upstream transcription factor 1, isoform CRA_a; USF 1; USF;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
67
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P22415 作为底物

Site PTM Type Enzyme
T100 Phosphorylation
T153 Phosphorylation P49840 (GSK3A) , Q16539 (MAPK14)
S186 Phosphorylation P49840 (GSK3A)
S189 Phosphorylation
K199 Acetylation
K214 Ubiquitination
K235 Acetylation
K235 Ubiquitination
K240 Ubiquitination
K246 Ubiquitination
S262 Phosphorylation P78527 (PRKDC)
K286 Ubiquitination
K288 Sumoylation
K288 Ubiquitination
K306 Sumoylation
S309 Phosphorylation

研究背景

功能:

Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.

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