产品: | 磷酸化 USF1 (Thr153) 抗体 |
货号: | AF8331 |
描述: | Rabbit polyclonal antibody to Phospho-USF1 (Thr153) |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Pig, Bovine, Rabbit, Dog |
分子量: | 34kDa; 34kD(Calculated). |
蛋白号: | P22415 |
RRID: | AB_2840393 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8331, RRID:AB_2840393.
展开/折叠
bHLHb11; Class B basic helix-loop-helix protein 11; FCHL 1; FCHL; FCHL1; HGNC:12593; HYPLIP 1; HYPLIP1; Major late transcription factor 1; Major late transcription factor; MLTF; MLTF I; MLTFI; Transcription factor USF-1; UEF; Upstream stimulatory factor 1; Upstream transcription factor 1; Upstream transcription factor 1, isoform CRA_a; USF 1; USF;
抗原和靶标
- P22415 USF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P22415 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T100 | Phosphorylation | Uniprot | |
T153 | Phosphorylation | P49840 (GSK3A) , Q16539 (MAPK14) | Uniprot |
S186 | Phosphorylation | P49840 (GSK3A) | Uniprot |
S189 | Phosphorylation | Uniprot | |
K199 | Acetylation | Uniprot | |
K214 | Ubiquitination | Uniprot | |
K235 | Acetylation | Uniprot | |
K235 | Ubiquitination | Uniprot | |
K240 | Ubiquitination | Uniprot | |
K246 | Ubiquitination | Uniprot | |
S262 | Phosphorylation | P78527 (PRKDC) | Uniprot |
K286 | Ubiquitination | Uniprot | |
K288 | Sumoylation | Uniprot | |
K288 | Ubiquitination | Uniprot | |
K306 | Sumoylation | Uniprot | |
S309 | Phosphorylation | Uniprot |
研究背景
Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Nucleus.
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a homodimer or a heterodimer (USF1/USF2). Interacts with varicella-zoster virus IE62 protein.
限制条款
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