Phospho-TNNI3 (Ser23+Ser24) Antibody - #AF8353
| 产品: | 磷酸化 TNNI3 (Ser23+Ser24) 抗体 |
| 货号: | AF8353 |
| 描述: | Rabbit polyclonal antibody to Phospho-TNNI3 (Ser23+Ser24) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 28kDa; 24kD(Calculated). |
| 蛋白号: | P19429 |
| RRID: | AB_2840415 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8353, RRID:AB_2840415.
展开/折叠
cardiac muscle; Cardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); Cardiomyopathy, familial hypertrophic, 7, included; CMD1FF; CMD2A; CMH7; cTnI; Familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; Tn1; Tni; TNN I3; TNNC 1; TNNC1; TNNI3; TNNI3_HUMAN; Troponin I; Troponin I cardiac; Troponin I cardiac muscle; Troponin I cardiac muscle isoform; Troponin I type 3 cardiac; troponin I, cardiac 3; TroponinI; Ttroponin I type 3 (cardiac);
抗原和靶标
- P19429 TNNI3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P19429 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Q13976 (PRKG1) , Q15139 (PRKD1) , P17612 (PRKACA) , Q05655 (PRKCD) , Q13131 (PRKAA1) , P17252 (PRKCA) | Uniprot |
| S24 | Phosphorylation | Q05655 (PRKCD) , P17612 (PRKACA) , P17252 (PRKCA) , Q15139 (PRKD1) , Q13976 (PRKG1) , Q13131 (PRKAA1) | Uniprot |
| Y26 | Phosphorylation | Uniprot | |
| Y29 | Phosphorylation | Uniprot | |
| T31 | Phosphorylation | Q13043 (STK4) | Uniprot |
| S39 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K40 | Methylation | Uniprot | |
| S42 | Phosphorylation | P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB) | Uniprot |
| S44 | Phosphorylation | P05771 (PRKCB) , P17252 (PRKCA) , Q02156 (PRKCE) , P05129 (PRKCG) | Uniprot |
| T51 | Phosphorylation | Q13043 (STK4) | Uniprot |
| K72 | Acetylation | Uniprot | |
| S77 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T78 | Phosphorylation | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Q13043 (STK4) | Uniprot |
| T143 | Phosphorylation | Q05655 (PRKCD) , Q13043 (STK4) , P05771 (PRKCB) , P17252 (PRKCA) | Uniprot |
| S150 | Phosphorylation | O75914 (PAK3) , Q13131 (PRKAA1) | Uniprot |
| S166 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K177 | Acetylation | Uniprot | |
| K178 | Acetylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | P17252 (PRKCA) | Uniprot |
研究背景
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T).
Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1.
Belongs to the troponin I family.
研究领域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Organismal Systems > Circulatory system > Cardiac muscle contraction. (View pathway)
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
限制条款
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