产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8358, RRID:AB_2840419.
展开/折叠
Abelson interactor 1; Abi-1; Abi1; ABI1_HUMAN; Abl binding protein 4; Abl interactor 1; Abl interactor protein 1; Abl-binding protein 4; AblBP4; e3B1; Eps8 binding protein; Eps8 SH3 domain binding protein; Eps8 SH3 domain-binding protein; Eps8-binding protein; Hssh3bp1; Interactor protein AblBP4; NAP1; Nap1 binding protein; Nap1-binding protein; Nap1BP; Spectrin SH3 domain binding protein 1; Spectrin SH3 domain-binding protein 1; SSH3BP;
抗原和靶标
- Q8IZP0 ABI1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELQMLLEEEIPSGKRALIESYQNLTRVADYCENNYIQATDKRKALEETKAYTTQSLASVAYQINALANNVLQLLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANMERPVRYIRKPIDYTVLDDVGHGVKWLKAKHGNNQPARTGTLSRTNPPTQKPPSPPMSGRGTLGRNTPYKTLEPVKPPTVPNDYMTSPARLGSQHSPGRTASLNQRPRTHSGSSGGSGSRENSGSSSIGIPIAVPTPSPPTIGPENISVPPPSGAPPAPPLAPLLPVSTVIAAPGSAPGSQYGTMTRQISRHNSTTSSTSSGGYRRTPSVTAQFSAQPHVNGGPLYSQNSISIAPPPPPMPQLTPQIPLTGFVARVQENIADSPTPPPPPPPDDIPMFDDSPPPPPPPPVDYEDEEAAVVQYNDPYADGDPAWAPKNYIEKVVAIYDYTKDKDDELSFMEGAIIYVIKKNDDGWYEGVCNRVTGLFPGNYVESIMHYTD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8IZP0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| Y23 | Phosphorylation | Uniprot | |
| Y37 | Phosphorylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| K123 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S187 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| T196 | Phosphorylation | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | P00519 (ABL1) | Uniprot |
| T215 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) , P27361 (MAPK3) | Uniprot |
| S222 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S225 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| T229 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S267 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| S319 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| Y333 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| Y355 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| T394 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S410 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| Y421 | Phosphorylation | Uniprot | |
| Y431 | Phosphorylation | Uniprot | |
| Y435 | Phosphorylation | Uniprot | |
| Y455 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| Y484 | Phosphorylation | Uniprot | |
| Y499 | Phosphorylation | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| Y506 | Phosphorylation | Uniprot | |
| T507 | Phosphorylation | Uniprot |
研究背景
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. Seems to be phosphorylated at Tyr-53 by ABL1, required for nuclear but not for synaptic localization.
Cytoplasm. Nucleus. Cell projection>Lamellipodium. Cell projection>Filopodium. Cell projection>Growth cone. Cell junction>Synapse>Postsynaptic density. Cytoplasm>Cytoskeleton.
Note: Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. May shuttle from the postsynaptic densities to the nucleus (By similarity).
Widely expressed, with highest expression in brain.
Interacts with ABL1, ENAH, STX1A, SNAP25, VAMP2, EPS8, and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1 (By similarity). Interacts with SOS1, SOS2, GRB2, SPTA1 and the first SH3 domain of NCK1. Isoform 6 does not interact with NCK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2. Interacts (via SH3 domain) with SHANK2 and SHANK3, but not SHANK1; the interaction is direct. Interacts with the heterodimer MYC:MAX; the interaction may enhance MYC:MAX transcriptional activity. Interacts with FNBP1L (via the SH3 domain), WASF2, and CDC42, but only in the presence of FNBP1L.
(Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL135.
The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.
Belongs to the ABI family.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.