| 产品: | 磷酸化 C5R1 (Ser334) 抗体 |
| 货号: | AF8362 |
| 描述: | Rabbit polyclonal antibody to Phospho-C5R1 (Ser334) |
| 应用: | WB |
| 反应: | Human, Rat, Monkey |
| 预测: | Horse, Rabbit |
| 分子量: | 39kDa; 39kD(Calculated). |
| 蛋白号: | P21730 |
| RRID: | AB_2840423 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8362, RRID:AB_2840423.
展开/折叠
ANAPHYLATOXIN RECEPTOR; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin receptor; C5A; C5a ligand; C5a-R; C5aR; C5AR_HUMAN; c5ar1; C5R1; CD88; CD88 antigen; Complement component 5 receptor 1; Complement component 5 receptor 2; Complement component 5a receptor 1;
抗原和靶标
- P21730 C5AR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRVPDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTINAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFGGAACSILPSLILLNMYASILLLATISADRFLLVFKPIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYRVVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLGFLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVVAVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLKKLDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSLPSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P21730 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y11 | Phosphorylation | Uniprot | |
| Y14 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S317 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T324 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S332 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S334 | Phosphorylation | Q05655 (PRKCD) , P17252 (PRKCA) , P05771-2 (PRKCB) , P05129 (PRKCG) | Uniprot |
| S338 | Phosphorylation | P17252 (PRKCA) | Uniprot |
研究背景
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.
Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist. Sulfation at Tyr-14 is important for CHIPS binding.
Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338.
Cell membrane>Multi-pass membrane protein. Cytoplasmic vesicle.
Note: Phosphorylated C5aR colocalizes with ARRB1 and ARRB2 in cytoplasmic vesicles.
Homodimer. May also form higher-order oligomers. Interacts (when phosphorylated) with ARRB1 and ARRB2; the interaction is associated with internalization of C5aR. Interacts (via N-terminal domain) with S.aureus chemotaxis inhibitory protein (CHIPS); the interaction blocks the receptor and may thus inhibit the immune response.
Belongs to the G-protein coupled receptor 1 family.
研究领域
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
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