Phospho-ASAP2 (Ser701) Antibody - #AF8367

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产品: 磷酸化 ASAP2 (Ser701) 抗体
货号: AF8367
描述: Rabbit polyclonal antibody to Phospho-ASAP2 (Ser701)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 112kDa; 112kD(Calculated).
蛋白号: O43150
RRID: AB_2840428

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-ASAP2 (Ser701) Antibody detects endogenous levels of ASAP2 only when phosphorylated at Ser701.
RRID:
AB_2840428
引用格式: Affinity Biosciences Cat# AF8367, RRID:AB_2840428.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AMAP 2; AMAP2; ANK repeat and PH domain-containing protein 2; Arf-GAP with SH3 domain; ArfGAP with SH3 domain, ankyrin repeat and PH domain 2; ASAP2; ASAP2_HUMAN; centaurin, beta 3; CENTB3; DDEF 2; DDEF2; Development and differentiation enhancing factor 2; Development and differentiation-enhancing factor 2; FLJ42910; KIAA0400; PAG 3; PAG3; Pap alpha; PAP; Paxillin associated protein with ARFGAP activity 3; Paxillin-associated protein with ARF GAP activity 3; Pyk2 C terminus associated protein; Pyk2 C-terminus-associated protein; SHAG 1; SHAG1;

抗原和靶标

免疫原:
Uniprot:

O43150(ASAP2_HUMAN) >>Visit HPA database.

基因/基因ID:
ASAP2(8853)
表达:
O43150 ASAP2_HUMAN:

Detected in heart, brain, placenta, kidney, monocytes and pancreas.

序列:
MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINSSGLAHVENEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVEQKEDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAKNIGNAGFNEIMECCLPAEDSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTRDIFGLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDESDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLARDAANLAKEKQRAFMPSILQNETYGALLSGSPPPAQPAAPSTTSAPPLPPRNVGKVQTASSANTLWKTNSVSVDGGSRQRSSSDPPAVHPPLPPLRVTSTNPLTPTPPPPVAKTPSVMEALSQPSKPAPPGISQIRPPPLPPQPPSRLPQKKPAPGADKSTPLTNKGQPRGPVDLSATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNCVADNPDELTFSEGDVIIVDGEEDQEWWIGHIDGDPGRKGAFPVSFVHFIAD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O43150 作为底物

Site PTM Type Enzyme
K81 Ubiquitination
Y201 Phosphorylation
K212 Ubiquitination
K242 Acetylation
K242 Ubiquitination
K274 Ubiquitination
K282 Ubiquitination
S285 Phosphorylation
Y293 Phosphorylation
S294 Phosphorylation
S311 Phosphorylation
K321 Acetylation
K325 Acetylation
S329 Phosphorylation
T336 Phosphorylation
S338 Phosphorylation
K405 Ubiquitination
K546 Ubiquitination
K555 Ubiquitination
T556 Phosphorylation
K662 Ubiquitination
Y689 Phosphorylation
S701 Phosphorylation
S712 Phosphorylation
S722 Phosphorylation
Y724 Phosphorylation
S728 Phosphorylation
Y763 Phosphorylation
S800 Phosphorylation
S809 Phosphorylation
S820 Phosphorylation
S822 Phosphorylation
T845 Phosphorylation
T900 Phosphorylation
T903 Phosphorylation

研究背景

功能:

Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.

翻译修饰:

Phosphorylated on tyrosine residues by SRC and PTK2B.

细胞定位:

Cytoplasm. Golgi apparatus>Golgi stack membrane>Peripheral membrane protein. Cell membrane>Peripheral membrane protein.
Note: Colocalizes with F-actin and ARF6 in phagocytic cups.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in heart, brain, placenta, kidney, monocytes and pancreas.

亚基结构:

Binds PXN, ARF1, ARF5, ARF6, PTK2B and SRC.

蛋白家族:

The conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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