产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8372, RRID:AB_2840433.
展开/折叠
Avian erythroblastosis virus E26 (v ets) oncogene homolog 1; C ets 1 protein; c-ets-1; ETS 1; Ets protein; ETS proto-oncogene 1, transcription factor; ETS1; ETS1 oncogene; ETS1 protein; ETS1_HUMAN; EWSR 2; EWSR2; FLJ10768; Oncogene ETS1; P54; Protein C-ets-1; v ets avian erythroblastosis virus E2 oncogene homolog; v ets avian erythroblastosis virus E2 oncogene homolog 1; v ets avian erythroblastosis virus E26 oncogene homolog 1; v ets erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1;
抗原和靶标
Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.
- P14921 ETS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKAAVDLKPTLTIIKTEKVDLELFPSPDMECADVPLLTPSSKEMMSQALKATFSGFTKEQQRLGIPKDPRQWTETHVRDWVMWAVNEFSLKGVDFQKFCMNGAALCALGKDCFLELAPDFVGDILWEHLEILQKEDVKPYQVNGVNPAYPESRYTSDYFISYGIEHAQCVPPSEFSEPSFITESYQTLHPISSEELLSLKYENDYPSVILRDPLQTDTLQNDYFAIKQEVVTPDNMCMGRTSRGKLGGQDSFESIESYDSCDRLTQSWSSQSSFNSLQRVPSYDSFDSEDYPAALPNHKPKGTFKDYVRDRADLNKDKPVIPAAALAGYTGSGPIQLWQFLLELLTDKSCQSFISWTGDGWEFKLSDPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQSLLGYTPEELHAMLDVKPDADE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P14921 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Acetylation | Uniprot | |
| K2 | Ubiquitination | Uniprot | |
| K8 | Acetylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Sumoylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| T38 | Phosphorylation | P28482 (MAPK1) , Q13164 (MAPK7) , P27361 (MAPK3) | Uniprot |
| S41 | Phosphorylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K138 | Sumoylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| Y158 | Phosphorylation | Uniprot | |
| K200 | Sumoylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| K245 | Acetylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S251 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S254 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| Y258 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| T265 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| Y283 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S288 | Phosphorylation | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K305 | Acetylation | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| K388 | Sumoylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| Y395 | Phosphorylation | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| T405 | Phosphorylation | Uniprot | |
| Y410 | Phosphorylation | Uniprot |
研究背景
Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.
Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
Cytoplasm. Nucleus.
Note: Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.
Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.
Binds DNA as a homodimer; homodimerization is required for transcription activation. Interacts with MAF and MAFB. Interacts with PAX5; the interaction alters DNA-binding properties (By similarity). Interacts with DAXX. Interacts with UBE2I. Interacts with SP100; the interaction is direct and modulates ETS1 transcriptional activity.
Belongs to the ETS family.
研究领域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
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