产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8379, RRID:AB_2840440.
展开/折叠
Heterogeneous nuclear ribonucleoprotein A0; hnRNA binding protein; hnRNP A0; HNRNPA0; HNRPA0; ROA0_HUMAN;
抗原和靶标
- Q13151 ROA0_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MENSQLCKLFIGGLNVQTSESGLRGHFEAFGTLTDCVVVVNPQTKRSRCFGFVTYSNVEEADAAMAASPHAVDGNTVELKRAVSREDSARPGAHAKVKKLFVGGLKGDVAEGDLIEHFSQFGTVEKAEIIADKQSGKKRGFGFVYFQNHDAADKAAVVKFHPIQGHRVEVKKAVPKEDIYSGGGGGGSRSSRGGRGGRGRGGGRDQNGLSKGGGGGYNSYGGYGGGGGGGYNAYGGGGGGSSYGGSDYGNGFGGFGSYSQHQSSYGPMKSGGGGGGGGSSWGGRSNSGPYRGGYGGGGGYGGSSF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13151 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S84 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| S88 | Phosphorylation | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Sumoylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K99 | Sumoylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K106 | Sumoylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K126 | Sumoylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K133 | Acetylation | Uniprot | |
| K133 | Sumoylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| K138 | Sumoylation | Uniprot | |
| R139 | Methylation | Uniprot | |
| Y145 | Phosphorylation | Uniprot | |
| K154 | Sumoylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K159 | Acetylation | Uniprot | |
| K159 | Sumoylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K171 | Sumoylation | Uniprot | |
| K172 | Sumoylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K176 | Sumoylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| R189 | Methylation | Uniprot | |
| R192 | Methylation | Uniprot | |
| R195 | Methylation | Uniprot | |
| R198 | Methylation | Uniprot | |
| R200 | Methylation | Uniprot | |
| R204 | Methylation | Uniprot | |
| K211 | Sumoylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| K269 | Sumoylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| R284 | Methylation | Uniprot | |
| R291 | Methylation | Uniprot | |
| S303 | Phosphorylation | Uniprot |
研究背景
mRNA-binding component of ribonucleosomes. Specifically binds AU-rich element (ARE)-containing mRNAs. Involved in post-transcriptional regulation of cytokines mRNAs.
Phosphorylated at Ser-84 by MAPKAPK2 in response to LPS treatment, promoting stabilization of GADD45A mRNA.
Arg-291 is dimethylated, probably to asymmetric dimethylarginine.
Nucleus.
Note: Component of ribonucleosomes.
限制条款
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