| 产品: | 磷酸化 CRMP-1 (Thr509) 抗体 |
| 货号: | AF8417 |
| 描述: | Rabbit polyclonal antibody to Phospho-CRMP-1 (Thr509) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 60~75kDa; 62kD(Calculated). |
| 蛋白号: | Q14194 |
| RRID: | AB_2840475 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8417, RRID:AB_2840475.
展开/折叠
Collapsin response mediator protein 1; CRMP 1; CRMP-1; Crmp1; Dihydropyrimidinase like 1; Dihydropyrimidinase related protein 1; Dihydropyrimidinase-related protein 1; DPYL1_HUMAN; DPYSL1; DRP 1; DRP-1; DRP1; ULIP-3; Ulip3; Unc-33-like phosphoprotein 3;
抗原和靶标
- Q14194 DPYL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSYQGKKSIPHITSDRLLIKGGRIINDDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDVNTYLQKPSQGMTAADDFFQGTRAALVGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVDITSWYDGVREELEVLVQDKGVNSFQVYMAYKDVYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRILEMGITGPEGHALSRPEELEAEAVFRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIAASLGTDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTLIPEGVNGIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITAKSHKSAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQGVSRGMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVAPPGGRSNITSLG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14194 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| S2 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| T101 | Phosphorylation | O14965 (AURKA) | Uniprot |
| T102 | Phosphorylation | O14965 (AURKA) | Uniprot |
| T253 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| K398 | Acetylation | Uniprot | |
| Y499 | Phosphorylation | Uniprot | |
| Y504 | Phosphorylation | P06241 (FYN) | Uniprot |
| T509 | Phosphorylation | P24941 (CDK2) , Q00535 (CDK5) , P49841 (GSK3B) | Uniprot |
| Y512 | Phosphorylation | Uniprot | |
| T514 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S518 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S521 | Phosphorylation | Uniprot | |
| S522 | Phosphorylation | Q00535 (CDK5) , Q92630 (DYRK2) | Uniprot |
| S524 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| T569 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot |
研究背景
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance. During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone. Involved in invasive growth and cell migration. May participate in cytokinesis.
Phosphorylation at Ser-522 enhances CRMP1-mediated alteration of FLNA ternary structure and FLNA dissociation from F-actin.
Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cell projection>Growth cone. Cytoplasm>Cytoskeleton. Perikaryon.
Note: Associated with centrosomes and the mitotic spindle during metaphase (PubMed:11562390). Colocalizes with FLNA and tubulin in the central region of DRG neuron growth cone (By similarity). Following SEMA3A stimulation of DRG neurons, colocalizes with F-actin (By similarity).
Brain.
Homotetramer, and heterotetramer with DPYSL2, DPYSL3, DPYSL4 or DPYSL5 (By similarity). Interacts with PLXNA1 (By similarity). Interacts with FLNA (via calponin-homology (CH) domain 1 and filamin repeat 24); the interaction alters FLNA ternary structure and thus promotes FLNA dissociation from F-actin.
Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.
限制条款
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