Phospho-LTK/ALK (Tyr672/Tyr1278) Antibody - #AF8442

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产品: 磷酸化 LTK/ALK (Tyr672/Tyr1278) 抗体
货号: AF8442
描述: Rabbit polyclonal antibody to Phospho-LTK/ALK (Tyr672/Tyr1278)
应用: WB
反应: Human, Mouse
预测: Horse, Rabbit, Chicken
分子量: 176KD; 92kD,176kD(Calculated).
蛋白号: P29376 | Q9UM73
RRID: AB_2840499

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 100ul RMB¥ 2800 现货
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Horse(100%), Rabbit(100%), Chicken(100%)
克隆:
Polyclonal
特异性:
Phospho-LTK/ALK (Tyr672/Tyr1278) Antibody detects endogenous levels of LTK/ALK only when phosphorylated at Tyr672/Tyr1278.
RRID:
AB_2840499
引用格式: Affinity Biosciences Cat# AF8442, RRID:AB_2840499.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Leukocyte receptor tyrosine kinase; Leukocyte tyrosine kinase; Leukocyte tyrosine kinase receptor; Ltk; LTK_HUMAN; Protein tyrosine kinase 1; TYK 1; TYK1; Alk; ALK tyrosine kinase receptor; ALK/EML4 fusion gene, included; ALK/NPM1 fusion gene, included; ALK_HUMAN; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; anaplastic lymphoma receptor tyrosine kinase; CD 246; CD246; CD246 antigen; EC 2.7.10.1; Ki 1; Ki1; mutant anaplastic lymphoma kinase; NBLST 3; NBLST3; Tcrz; TFG/ALK;

抗原和靶标

免疫原:
Uniprot:

P29376(LTK_HUMAN) >>Visit HPA database.

Q9UM73(ALK_HUMAN) >>Visit HPA database.

基因/基因ID:
LTK(4058) | ALK(238)
表达:
P29376 LTK_HUMAN:

Expressed in non-hematopoietic cell lines and T- and B-cell lines.

Q9UM73 ALK_HUMAN:

Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

序列:
MGCWGQLLVWFGAAGAILCSSPGSQETFLRSSPLPLASPSPRDPKVSAPPSILEPASPLNSPGTEGSWLFSTCGASGRHGPTQTQCDGAYAGTSVVVTVGAAGQLRGVQLWRVPGPGQYLISAYGAAGGKGAKNHLSRAHGVFVSAIFSLGLGESLYILVGQQGEDACPGGSPESQLVCLGESRAVEEHAAMDGSEGVPGSRRWAGGGGGGGGATYVFRVRAGELEPLLVAAGGGGRAYLRPRDRGRTQASPEKLENRSEAPGSGGRGGAAGGGGGWTSRAPSPQAGRSLQEGAEGGQGCSEAWATLGWAAAGGFGGGGGACTAGGGGGGYRGGDASETDNLWADGEDGVSFIHPSSELFLQPLAVTENHGEVEIRRHLNCSHCPLRDCQWQAELQLAECLCPEGMELAVDNVTCMDLHKPPGPLVLMVAVVATSTLSLLMVCGVLILVKQKKWQGLQEMRLPSPELELSKLRTSAIRTAPNPYYCQVGLGPAQSWPLPPGVTEVSPANVTLLRALGHGAFGEVYEGLVIGLPGDSSPLQVAIKTLPELCSPQDELDFLMEALIISKFRHQNIVRCVGLSLRATPRLILLELMSGGDMKSFLRHSRPHLGQPSPLVMRDLLQLAQDIAQGCHYLEENHFIHRDIAARNCLLSCAGPSRVAKIGDFGMARDIYRASYYRRGDRALLPVKWMPPEAFLEGIFTSKTDSWSFGVLLWEIFSLGYMPYPGRTNQEVLDFVVGGGRMDPPRGCPGPVYRIMTQCWQHEPELRPSFASILERLQYCTQDPDVLNSLLPMELGPTPEEEGTSGLGNRSLECLRPPQPQELSPEKLKSWGGSPLGPWLSSGLKPLKSRGLQPQNLWNPTYRS

MGAIGLLWLLPLLLSTAAVGSGMGTGQRAGSPAAGPPLQPREPLSYSRLQRKSLAVDFVVPSLFRVYARDLLLPPSSSELKAGRPEARGSLALDCAPLLRLLGPAPGVSWTAGSPAPAEARTLSRVLKGGSVRKLRRAKQLVLELGEEAILEGCVGPPGEAAVGLLQFNLSELFSWWIRQGEGRLRIRLMPEKKASEVGREGRLSAAIRASQPRLLFQIFGTGHSSLESPTNMPSPSPDYFTWNLTWIMKDSFPFLSHRSRYGLECSFDFPCELEYSPPLHDLRNQSWSWRRIPSEEASQMDLLDGPGAERSKEMPRGSFLLLNTSADSKHTILSPWMRSSSEHCTLAVSVHRHLQPSGRYIAQLLPHNEAAREILLMPTPGKHGWTVLQGRIGRPDNPFRVALEYISSGNRSLSAVDFFALKNCSEGTSPGSKMALQSSFTCWNGTVLQLGQACDFHQDCAQGEDESQMCRKLPVGFYCNFEDGFCGWTQGTLSPHTPQWQVRTLKDARFQDHQDHALLLSTTDVPASESATVTSATFPAPIKSSPCELRMSWLIRGVLRGNVSLVLVENKTGKEQGRMVWHVAAYEGLSLWQWMVLPLLDVSDRFWLQMVAWWGQGSRAIVAFDNISISLDCYLTISGEDKILQNTAPKSRNLFERNPNKELKPGENSPRQTPIFDPTVHWLFTTCGASGPHGPTQAQCNNAYQNSNLSVEVGSEGPLKGIQIWKVPATDTYSISGYGAAGGKGGKNTMMRSHGVSVLGIFNLEKDDMLYILVGQQGEDACPSTNQLIQKVCIGENNVIEEEIRVNRSVHEWAGGGGGGGGATYVFKMKDGVPVPLIIAAGGGGRAYGAKTDTFHPERLENNSSVLGLNGNSGAAGGGGGWNDNTSLLWAGKSLQEGATGGHSCPQAMKKWGWETRGGFGGGGGGCSSGGGGGGYIGGNAASNNDPEMDGEDGVSFISPLGILYTPALKVMEGHGEVNIKHYLNCSHCEVDECHMDPESHKVICFCDHGTVLAEDGVSCIVSPTPEPHLPLSLILSVVTSALVAALVLAFSGIMIVYRRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLKEVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQDELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLRETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTCPGPGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSKTDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVYRIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEEKVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAAEISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGSWFTEKPTKKNNPIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEPSSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTILKSKNSMNQPGP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Chicken
100
Rabbit
100
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P29376/Q9UM73 作为底物

Site PTM Type Enzyme
Y46 Phosphorylation
S47 Phosphorylation
S62 Phosphorylation
S211 Phosphorylation
S312 Phosphorylation
S319 Phosphorylation
S326 Phosphorylation
S329 Phosphorylation
Y705 Phosphorylation
S1075 Phosphorylation
Y1078 Phosphorylation
T1090 Phosphorylation
Y1092 Phosphorylation
Y1096 Phosphorylation
Y1131 Phosphorylation
Y1278 Phosphorylation Q9UM73 (ALK)
Y1282 Phosphorylation Q9UM73 (ALK)
Y1283 Phosphorylation Q9UM73 (ALK)
Y1359 Phosphorylation
Y1401 Phosphorylation
K1451 Methylation
K1455 Methylation
S1481 Phosphorylation
S1495 Phosphorylation
T1506 Phosphorylation
Y1507 Phosphorylation
S1509 Phosphorylation
K1517 Ubiquitination
K1518 Ubiquitination
Y1584 Phosphorylation
Y1586 Phosphorylation
T1597 Phosphorylation
Y1604 Phosphorylation Q9UM73 (ALK)
K1610 Methylation
Site PTM Type Enzyme
S259 Phosphorylation
S475 Phosphorylation
Y672 Phosphorylation
Y676 Phosphorylation
Y677 Phosphorylation

翻译修饰 - P29376/Q9UM73 作为激酶

Substrate Site Source
P14618 (PKM) Y105 Uniprot
P23246 (SFPQ) Y293 Uniprot
P31939 (ATIC) Y104 Uniprot
P40763 (STAT3) Y705 Uniprot
P42768 (WAS) Y102 Uniprot
P49841 (GSK3B) S9 Uniprot
P50552 (VASP) Y39 Uniprot
P62993 (GRB2) Y160 Uniprot
Q06124 (PTPN11) Y546 Uniprot
Q06124 (PTPN11) Y584 Uniprot
Q86WB0 (ZC3HC1) Y105 Uniprot
Q86WB0 (ZC3HC1) Y137 Uniprot
Q9C0H9-5 (SRCIN1) Y396 Uniprot
Q9UM73 (ALK) Y1278 Uniprot
Q9UM73 (ALK) Y1282 Uniprot
Q9UM73 (ALK) Y1283 Uniprot
Q9UM73 (ALK) Y1604 Uniprot
Substrate Site Source

研究背景

功能:

Receptor with a tyrosine-protein kinase activity. The exact function of this protein is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport.

细胞定位:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in non-hematopoietic cell lines and T- and B-cell lines.

亚基结构:

Homodimer when bound to ligand (Probable). Part of a complex including LTK, TNK2 and GRB2, in which GRB2 promotes LTK recruitment by TNK2.

蛋白家族:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

功能:

Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.

翻译修饰:

Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association.

N-glycosylated.

细胞定位:

Cell membrane>Single-pass type I membrane protein.
Note: Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells.

亚基结构:

Homodimer. Homodimerizes when bound to ligand. Interacts with FRS2, IRS1, MDK, PTN and SHC1. Interacts with CBL, PIK3R1 and PLCG1 (By similarity).

蛋白家族:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

研究领域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

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