Phospho-MYPT1/2 (Ser668/Ser618) Antibody - #AF8447

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产品: 磷酸化 MYPT1/2 (Ser668/Ser618) 抗体
货号: AF8447
描述: Rabbit polyclonal antibody to Phospho-MYPT1/2 (Ser668/Ser618)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 115KD; 115kD,110kD(Calculated).
蛋白号: O14974 | O60237
RRID: AB_2840504

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-MYPT1/2 (Ser668/Ser618) Antibody detects endogenous levels of MYPT1/2 only when phosphorylated at Ser668/Ser618.
RRID:
AB_2840504
引用格式: Affinity Biosciences Cat# AF8447, RRID:AB_2840504.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

M130; MBS; Myosin binding subunit; Myosin phosphatase target subunit 1; Myosin phosphatase targeting subunit 1; Myosin phosphatase-targeting subunit 1; MYPT1; MYPT1_HUMAN; PPP1R12A; Protein phosphatase 1 regulatory inhibitor subunit 12A; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase 1, regulatory (inhibitor) subunit 12A; Protein phosphatase myosin binding subunit; Protein phosphatase myosin-binding subunit; Myosin Phosphatase 2; Myosin phosphatase regulatory subunit; Myosin phosphatase targeting subunit 2; Myosin phosphatase, target subunit 2; MYPT2; PP1bp55; PPP1R12B; Protein phosphatase 1 regulatory subunit 12B; Protein phosphatase 1, regulatory (inhibitor) subunit 12B;

抗原和靶标

免疫原:
Uniprot:

O14974(MYPT1_HUMAN) >>Visit HPA database.

O60237(MYPT2_HUMAN) >>Visit HPA database.

基因/基因ID:
PPP1R12A(4659) | PPP1R12B(4660)
表达:
O14974 MYPT1_HUMAN:

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

O60237 MYPT2_HUMAN:

Detected in skeletal muscle, fetal and adult heart, brain, placenta, kidney, spleen, thymus, pancreas and lung. Isoform 3 and isoform 4 are heart specific.

序列:
MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANMDNNQSQKTFKNKETLIIEPEKNASRIESLEQEKVDEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPLASVTNANTSSTQAAPVAVTTPTVSSGQATPTSPIKKFPTTATKISPKEEERKDESPATWRLGLRKTGSYGALAEITASKEGQKEKDTAGVTRSASSPRLSSSLDNKEKEKDSKGTRLAYVAPTIPRRLASTSDIEEKENRDSSSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHKSCSFGRRQDDLISSSVPSTTSTPTVTSAAGLQKSLLSSTSTTTKITTGSSSAGTQSSTSNRLWAEDSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTTEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETSREDEYKQKYSRTYDETYQRYRPVSTSSSTTPSSSLSTMSSSLYASSQLNRPNSLVGITSAYSRGITKENEREGEKREEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSAGDRYDSLLGRSGSYSYLEERKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK

MAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGAVFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSSSSEEEEGEDEASESETEKEADKKPEAFVNHSNSESKSSITEQIPAPAQNTFSASSARRFSSGLFNKPEEPKDESPSSWRLGLRKTGSHNMLSEVANSREPIRDRGSSIYRSSSSPRISALLDNKDKERENKSYISSLAPRKLNSTSDIEEKENRESAVNLVRSGSYTRQLWRDEAKGNEIPQTIAPSTYVSTYLKRTPHKSQADTTAEKTADNVSSSTPLCVITNRPLPSTANGVTATPVLSITGTDSSVEAREKRRSYLTPVRDEEAESLRKARSRQARQTRRSTQGVTLTDLQEAERTFSRSRAERQAQEQPREKPTDTEGLEGSPEKHEPSAVPATEAGEGQQPWGRSLDEEPICHRLRCPAQPDKPTTPASPSTSRPSLYTSSHLLWTNRFSVPDSESSETTTNTTTAKEMDKNENEEADLDEQSSKRLSIRERRRPKERRRGTGINFWTKDEDETDGSEEVKETWHERLSRLESGGSNPTTSDSYGDRASARARREAREARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKSKLEKVAQQKQEKTSDRSSVLEMEKRERRALERKMSEMEEEMKVLTELKSDNQRLKDENGALIRVISKLSK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O14974/O60237 作为底物

Site PTM Type Enzyme
K9 Acetylation
S20 Phosphorylation
T22 Phosphorylation
T34 Phosphorylation
Y68 Phosphorylation
T234 Phosphorylation
K244 Ubiquitination
K285 Ubiquitination
K286 Ubiquitination
S292 Phosphorylation
S299 Phosphorylation
S304 Phosphorylation
S313 Phosphorylation
S336 Phosphorylation
S365 Phosphorylation
T406 Phosphorylation
T408 Phosphorylation
S409 Phosphorylation
T416 Phosphorylation
T417 Phosphorylation
T419 Phosphorylation
S422 Phosphorylation
S432 Phosphorylation P06493 (CDK1)
K442 Acetylation
T443 Phosphorylation O75116 (ROCK2)
S445 Phosphorylation O60285 (NUAK1) , O95835 (LATS1)
Y446 Phosphorylation
T453 Phosphorylation
K456 Acetylation
K460 Acetylation
T464 Phosphorylation
S470 Phosphorylation
S472 Phosphorylation O60285 (NUAK1)
S473 Phosphorylation P06493 (CDK1)
S477 Phosphorylation
S478 Phosphorylation
S479 Phosphorylation
S489 Phosphorylation
Y496 Phosphorylation
T500 Phosphorylation
S507 Phosphorylation
T508 Phosphorylation
S509 Phosphorylation
S520 Phosphorylation
S525 Phosphorylation
T529 Phosphorylation
K539 Methylation
S541 Phosphorylation
S542 Phosphorylation
S547 Phosphorylation
T548 Phosphorylation
Y549 Phosphorylation
K551 Methylation
S552 Phosphorylation
S554 Phosphorylation
T571 Phosphorylation
T575 Phosphorylation
S578 Phosphorylation
S585 Phosphorylation
S601 Phosphorylation P06493 (CDK1)
S607 Phosphorylation
S608 Phosphorylation
T609 Phosphorylation
S618 Phosphorylation
T631 Phosphorylation
T645 Phosphorylation
T647 Phosphorylation
T652 Phosphorylation
S668 Phosphorylation
Y669 Phosphorylation
T671 Phosphorylation
S678 Phosphorylation
S680 Phosphorylation
S692 Phosphorylation Q13976 (PRKG1)
S695 Phosphorylation P17612 (PRKACA) , Q13976 (PRKG1)
T696 Phosphorylation Q09013 (DMPK) , P04049 (RAF1) , Q13464 (ROCK1) , P17612 (PRKACA) , O75116 (ROCK2) , Q13418 (ILK)
T700 Phosphorylation
T702 Phosphorylation
T710 Phosphorylation Q13418 (ILK)
S716 Phosphorylation
T717 Phosphorylation
T719 Phosphorylation
K743 Acetylation
T748 Phosphorylation
S749 Phosphorylation
Y754 Phosphorylation
K755 Acetylation
Y758 Phosphorylation
S759 Phosphorylation
T761 Phosphorylation
Y762 Phosphorylation
T765 Phosphorylation
Y766 Phosphorylation
Y769 Phosphorylation
S781 Phosphorylation
S790 Phosphorylation
Y792 Phosphorylation
S794 Phosphorylation
S795 Phosphorylation
S802 Phosphorylation
Y810 Phosphorylation
S811 Phosphorylation
S839 Phosphorylation
S852 Phosphorylation P17612 (PRKACA) , O75116 (ROCK2) , Q13976 (PRKG1) , Q9Y5S2 (CDC42BPB) , Q13464 (ROCK1)
T853 Phosphorylation P17612 (PRKACA) , Q13464 (ROCK1) , Q04759 (PRKCQ)
S856 Phosphorylation
T859 Phosphorylation
S862 Phosphorylation
S871 Phosphorylation
T873 Phosphorylation
S877 Phosphorylation
T884 Phosphorylation
S886 Phosphorylation
S888 Phosphorylation
Y890 Phosphorylation
T892 Phosphorylation
S893 Phosphorylation
S894 Phosphorylation
S896 Phosphorylation
Y901 Phosphorylation
S903 Phosphorylation
S908 Phosphorylation
S910 Phosphorylation O60285 (NUAK1)
Y911 Phosphorylation
S912 Phosphorylation
Y913 Phosphorylation
Y920 Phosphorylation
S921 Phosphorylation
S922 Phosphorylation
Y936 Phosphorylation
K945 Ubiquitination
S978 Phosphorylation
S995 Phosphorylation
K1002 Methylation
K1008 Methylation
S1029 Phosphorylation
Site PTM Type Enzyme
S29 Phosphorylation
T31 Phosphorylation
S67 Phosphorylation
K303 Ubiquitination
Y340 Phosphorylation
S347 Phosphorylation
S358 Phosphorylation
S359 Phosphorylation
S374 Phosphorylation
T376 Phosphorylation
T410 Phosphorylation
S420 Phosphorylation
S434 Phosphorylation
T445 Phosphorylation
S447 Phosphorylation
S452 Phosphorylation
S471 Phosphorylation
S472 Phosphorylation
S473 Phosphorylation
S474 Phosphorylation
S492 Phosphorylation
Y493 Phosphorylation
S504 Phosphorylation
S506 Phosphorylation
S523 Phosphorylation
S525 Phosphorylation
T527 Phosphorylation
Y549 Phosphorylation
S561 Phosphorylation
T565 Phosphorylation
S618 Phosphorylation
Y619 Phosphorylation
T621 Phosphorylation
T642 Phosphorylation
S645 Phosphorylation
T646 Phosphorylation Q13464 (ROCK1)
T650 Phosphorylation
T652 Phosphorylation
S687 Phosphorylation
S711 Phosphorylation
S735 Phosphorylation
Y744 Phosphorylation
S760 Phosphorylation
S762 Phosphorylation
S789 Phosphorylation
S790 Phosphorylation
S794 Phosphorylation
T808 Phosphorylation
S835 Phosphorylation
S839 Phosphorylation
S842 Phosphorylation
T845 Phosphorylation
T846 Phosphorylation
S847 Phosphorylation
S849 Phosphorylation
S855 Phosphorylation
T869 Phosphorylation
S878 Phosphorylation
S947 Phosphorylation
S981 Phosphorylation

研究背景

功能:

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

翻译修饰:

Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.

细胞定位:

Cytoplasm. Cytoplasm>Cytoskeleton>Stress fiber.
Note: Also along actomyosin filaments.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

亚基结构:

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16. Interacts directly with PRKG1. Non-covalent dimer of 2 dimers; PRKG1-PRKG1 and PPP1R12A-PPP1R12A. Interacts with SMTNL1 (By similarity). Interacts with PPP1CB; the interaction is direct. Interacts (when phosphorylated at Ser-445, Ser-472 and Ser-910) with 14-3-3. Interacts with ROCK1 and ROCK2. Interacts with isoform 1 and isoform 2 of ZIPK/DAPK3. Interacts with RAF1. Interacts with HIF1AN. Interacts with NCKAP1L.

蛋白家族:

Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.

The KVKF motif mediates interaction with PPP1CB.

功能:

Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus.

细胞定位:

Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber.
Note: Along actomyosin filaments.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in skeletal muscle, fetal and adult heart, brain, placenta, kidney, spleen, thymus, pancreas and lung. Isoform 3 and isoform 4 are heart specific.

亚基结构:

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12B mediates binding to myosin. Isoform 3 and isoform 4 bind PPP1R12A, but not isoform 1 of PPP1R12B itself. Binds IL16.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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