| 产品: | 磷酸化 BCL-3 (Ser402) 抗体 |
| 货号: | AF8466 |
| 描述: | Rabbit polyclonal antibody to Phospho-BCL-3 (Ser402) |
| 应用: | WB IHC |
| 反应: | Human |
| 预测: | Horse |
| 分子量: | 50-60kD; 48kD(Calculated). |
| 蛋白号: | P20749 |
| RRID: | AB_2840520 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8466, RRID:AB_2840520.
展开/折叠
B cell CLL/lymphoma 3; B cell leukemia/lymphoma 3; B cell lymphoma 3 encoded protein; B-cell lymphoma 3 protein; Bcl 3; BCL 4; BCL-3; Bcl3; BCL3_HUMAN; BCL4; Chronic lymphatic leukemia protein; D19S37; Protein Bcl 3; Proto-oncogene BCL3;
抗原和靶标
- P20749 BCL3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRCPAGAMDEGPVDLRTRPKAAGLPGAALPLRKRPLRAPSPEPAAPRGAAGLVVPLDPLRGGCDLPAVPGPPHGLARPEALYYPGALLPLYPTRAMGSPFPLVNLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P20749 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K21 | Ubiquitination | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| K338 | Methylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | P49840 (GSK3A) | Uniprot |
| S406 | Phosphorylation | P49840 (GSK3A) | Uniprot |
| S448 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot |
研究背景
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity).
Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation (By similarity).
Activated by phosphorylation.
Nucleus. Cytoplasm. Cytoplasm>Perinuclear region.
Note: Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
Component of a complex consisting of the NF-kappa-B p52-p52 homodimer and BCL3. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Interacts with N4BP2, COPS5 and PIR. Interacts with CYLD (By similarity).
研究领域
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
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