Phospho-CD19 (Tyr500) Antibody - #AF8467
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8467, RRID:AB_2840521.
展开/折叠
B lymphocyte antigen CD19; CD19 molecule; CD19 antigen; Differentiation antigen CD19; Ig kappa chain V-I region HK101; IGKC; Immunoglobulin kappa constant; immunoglobulin kappa light chain;
抗原和靶标
Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:2463100, PubMed:16672701).
- P15391 CD19_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPPPRLLFFLLFLTPMEVRPEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWKVSAVTLAYLIFCLCSLVGILHLQRALVLRRKRKRMTDPTRRFFKVTPPPGSGPQNQYGNVLSLPTPTSGLGRAQRWAAGLGGTAPSYGNPSSDVQADGALGSRSPPGVGPEEEEGEGYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEDEPLGPEDEDSFSNAESYENEDEELTQPVARTMDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSIRGQPGPNHEEDADSYENMDNPDGPDPAWGGGGRMGTWSTR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15391 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N86 | N-Glycosylation | Uniprot | |
| N125 | N-Glycosylation | Uniprot | |
| T337 | Phosphorylation | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| Y378 | Phosphorylation | Uniprot | |
| Y409 | Phosphorylation | P07948 (LYN) | Uniprot |
| Y439 | Phosphorylation | Uniprot | |
| T475 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| Y500 | Phosphorylation | P07948 (LYN) | Uniprot |
| Y508 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S515 | Phosphorylation | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| Y531 | Phosphorylation | P07948 (LYN) | Uniprot |
研究背景
Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge.
Phosphorylated on tyrosine following B-cell activation. Phosphorylated on tyrosine residues by LYN. Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity).
Cell membrane>Single-pass type I membrane protein. Membrane raft>Single-pass type I membrane protein.
Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level).
Interacts with CR2/CD21. Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells. Interacts directly with CD81 (via the second extracellular domain); this interaction is initiated early during biosynthesis in the ER/pre-Golgi compartments and is essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Interacts with LYN. Interacts (when tyrosine phosphorylated) with the regulatory p85 subunit of phosphatidylinositol 3-kinase (PIK3R1 or PIK3R2).
研究领域
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
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