Phospho-EIF5 (Ser389+Ser390) Antibody - #AF8473
| 产品: | 磷酸化 EIF5 (Ser389+Ser390) 抗体 |
| 货号: | AF8473 |
| 描述: | Rabbit polyclonal antibody to Phospho-EIF5 (Ser389+Ser390) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 49kDa; 49kD(Calculated). |
| 蛋白号: | P55010 |
| RRID: | AB_2840527 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF8473, RRID:AB_2840527.
展开/折叠
2810011H21Rik; D12Ertd549e; EIF 5; EIF 5A; eIF-5; Eif5; Eukaryotic initiation factor 5; Eukaryotic translation initiation factor 5; IF5_HUMAN; MGC36374; MGC36509;
抗原和靶标
- P55010 IF5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P55010 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| Y14 | Phosphorylation | Uniprot | |
| Y16 | Phosphorylation | Uniprot | |
| K24 | Acetylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| K70 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | P67870 (CSNK2B) , P68400 (CSNK2A1) | Uniprot |
| S175 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T208 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S220 | Phosphorylation | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| Y362 | Phosphorylation | Uniprot | |
| K369 | Acetylation | Uniprot | |
| S389 | Phosphorylation | P67870 (CSNK2B) , P68400 (CSNK2A1) | Uniprot |
| S390 | Phosphorylation | P68400 (CSNK2A1) , P67870 (CSNK2B) | Uniprot |
| Y405 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| T416 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot |
研究背景
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Interacts with FMR1 isoform 6; this interaction occurs in a RNA-dependent manner.
Belongs to the eIF-2-beta/eIF-5 family.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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