产品: 磷酸化 EIF5 (Ser389+Ser390) 抗体
货号: AF8473
描述: Rabbit polyclonal antibody to Phospho-EIF5 (Ser389+Ser390)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 49kDa; 49kD(Calculated).
蛋白号: P55010
RRID: AB_2840527

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(90%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-EIF5 (Ser389+Ser390) Antibody detects endogenous levels of EIF5 only when phosphorylated at Ser389+Ser390.
RRID:
AB_2840527
引用格式: Affinity Biosciences Cat# AF8473, RRID:AB_2840527.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2810011H21Rik; D12Ertd549e; EIF 5; EIF 5A; eIF-5; Eif5; Eukaryotic initiation factor 5; Eukaryotic translation initiation factor 5; IF5_HUMAN; MGC36374; MGC36509;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
90
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P55010 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
S8 Phosphorylation
S10 Phosphorylation
Y14 Phosphorylation
Y16 Phosphorylation
K24 Acetylation
K24 Ubiquitination
K28 Acetylation
K33 Acetylation
K55 Acetylation
K55 Ubiquitination
K70 Ubiquitination
K95 Ubiquitination
K114 Ubiquitination
K115 Ubiquitination
S121 Phosphorylation
T139 Phosphorylation
S149 Phosphorylation
S151 Phosphorylation
S174 Phosphorylation P67870 (CSNK2B) , P68400 (CSNK2A1)
S175 Phosphorylation
S176 Phosphorylation
T178 Phosphorylation
T207 Phosphorylation P68400 (CSNK2A1)
T208 Phosphorylation P68400 (CSNK2A1)
S220 Phosphorylation
T227 Phosphorylation
S229 Phosphorylation
K261 Ubiquitination
K288 Ubiquitination
Y362 Phosphorylation
K369 Acetylation
S389 Phosphorylation P67870 (CSNK2B) , P68400 (CSNK2A1)
S390 Phosphorylation P68400 (CSNK2A1) , P67870 (CSNK2B)
Y405 Phosphorylation
S406 Phosphorylation
S410 Phosphorylation
T416 Phosphorylation
S419 Phosphorylation

研究背景

功能:

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

亚基结构:

Interacts with FMR1 isoform 6; this interaction occurs in a RNA-dependent manner.

蛋白家族:

Belongs to the eIF-2-beta/eIF-5 family.

研究领域

· Genetic Information Processing > Translation > RNA transport.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.