Phospho-GFAP (Ser13) Antibody - #AF8479

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产品: 磷酸化 GFAP (Ser13) 抗体
货号: AF8479
描述: Rabbit polyclonal antibody to Phospho-GFAP (Ser13)
应用: WB IF/ICC
反应: Human
预测: Pig, Bovine, Horse, Rabbit
分子量: 50kDa; 50kD(Calculated).
蛋白号: P14136
RRID: AB_2840533

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(90%), Horse(90%), Rabbit(80%)
克隆:
Polyclonal
特异性:
Phospho-GFAP (Ser13) Antibody detects endogenous levels of GFAP only when phosphorylated at Ser13.
RRID:
AB_2840533
引用格式: Affinity Biosciences Cat# AF8479, RRID:AB_2840533.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

wu:fb34h11; ALXDRD; cb345; etID36982.3; FLJ42474; FLJ45472; GFAP; GFAP_HUMAN; gfapl; Glial fibrillary acidic protein; Intermediate filament protein; wu:fk42c12; xx:af506734; zgc:110485;

抗原和靶标

免疫原:
Uniprot:

P14136(GFAP_HUMAN) >>Visit HPA database.

基因/基因ID:
GFAP(2670)
表达:
P14136 GFAP_HUMAN:

Expressed in cells lacking fibronectin.

序列:
MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
90
Bovine
90
Rabbit
80
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P14136 作为底物

Site PTM Type Enzyme
T7 Phosphorylation Q96GD4 (AURKB) , P17612 (PRKACA) , Q13464 (ROCK1)
S8 Phosphorylation P17612 (PRKACA)
R11 Methylation
R12 Methylation
S13 Phosphorylation Q13464 (ROCK1) , P17612 (PRKACA) , Q96GD4 (AURKB)
Y14 Phosphorylation
S17 Phosphorylation
S38 Phosphorylation Q96GD4 (AURKB) , Q13464 (ROCK1) , P17612 (PRKACA)
K95 Acetylation
Y116 Phosphorylation
T131 Phosphorylation
T150 Phosphorylation
K154 Acetylation
Y172 Phosphorylation
K189 Acetylation
K228 Acetylation
T240 Phosphorylation
Y242 Phosphorylation
K260 Acetylation
S305 Phosphorylation
Y324 Phosphorylation
K339 Acetylation
Y349 Phosphorylation

研究背景

功能:

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

翻译修饰:

Phosphorylated by PKN1.

细胞定位:

Cytoplasm.
Note: Associated with intermediate filaments.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in cells lacking fibronectin.

亚基结构:

Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).

蛋白家族:

Belongs to the intermediate filament family.

研究领域

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

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