Phospho-CBX1 / HP1 beta (Thr51) Antibody - #AF8483

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产品: 磷酸化 CBX1 / HP1 beta (Thr51) 抗体
货号: AF8483
描述: Rabbit polyclonal antibody to Phospho-CBX1 / HP1 beta (Thr51)
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 25 kDa; 21kD(Calculated).
蛋白号: P83916
RRID: AB_2840537

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
克隆:
Polyclonal
特异性:
Phospho-CBX1 / HP1 beta (Thr51) Antibody detects endogenous levels of CBX1 / HP1 beta only when phosphorylated at Thr51.
RRID:
AB_2840537
引用格式: Affinity Biosciences Cat# AF8483, RRID:AB_2840537.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CBX 1; CBX; Cbx1; CBX1_HUMAN; Chromobox 1; Chromobox homolog 1 (HP1 beta homolog Drosophila ); chromobox homolog 1; Chromobox protein homolog 1; Drosophila HP1 beta; Heterochromatin protein 1 beta; Heterochromatin protein 1 homolog beta; Heterochromatin protein p25; heterochromatin protein p25 beta; HP1 beta; HP1 beta homolog; HP1 beta homolog Drosophila; HP1, Drosophila. homolog of, beta; HP1beta; HP1Hs beta; HP1Hsbeta; Human heterochromatin protein p25 mRNA complete cds; M31; MOD 1; MOD1; Modifier 1 protein; p25beta;

抗原和靶标

免疫原:
Uniprot:

P83916(CBX1_HUMAN) >>Visit HPA database.

基因/基因ID:
CBX1(10951)
表达:
P83916 CBX1_HUMAN:

Expressed in all adult and embryonic tissues.

序列:
MGKKQNKKKVEEVLEEEEEEYVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESKPKKKKEESEKPRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPSEDDDKKDDKN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P83916 作为底物

Site PTM Type Enzyme
K9 Acetylation
K9 Methylation
K9 Ubiquitination
Y21 Phosphorylation
K25 Ubiquitination
K33 Acetylation
K35 Acetylation
K35 Methylation
K35 Ubiquitination
K41 Acetylation
K41 Ubiquitination
K43 Ubiquitination
T51 Phosphorylation P68400 (CSNK2A1)
S70 Phosphorylation
K72 Ubiquitination
T73 Phosphorylation
T77 Phosphorylation
S80 Phosphorylation
K84 Acetylation
K84 Ubiquitination
S89 Phosphorylation
S91 Phosphorylation
S98 Phosphorylation
K104 Ubiquitination
K109 Ubiquitination
R111 Methylation
R115 Methylation
S128 Phosphorylation
S129 Phosphorylation
K139 Acetylation
K139 Ubiquitination
K150 Ubiquitination
K155 Ubiquitination
C156 S-Nitrosylation
S172 Phosphorylation
Y173 Phosphorylation
S175 Phosphorylation
K180 Ubiquitination
K181 Ubiquitination
K184 Ubiquitination

研究背景

功能:

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.

翻译修饰:

Not phosphorylated.

Ubiquitinated.

细胞定位:

Nucleus.
Note: Unassociated with chromosomes during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in all adult and embryonic tissues.

亚基结构:

Homodimer (By similarity). Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain. Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain (By similarity). Interacts with SUV39H1 and SETDB1, KMT5B and KMT5C. Interacts with PRDM6 (By similarity). Interacts with POGZ. Interacts with CHAMP1. Interacts with INCENP. Interacts with SGO1; the CBX1 homodimer binds to one molecule of SGO1. Interacts with LRIF1 (via PxVxL motif).

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